Paenibacillus puldeungensis sp. nov., isolated from a grassy sandbank

Abstract

The taxonomic position of a Gram-stain-positive, rod-shaped, endospore-forming, facultatively anaerobic bacterial strain, CAU 9324(T), isolated from a grassy sandbank was investigated by using a polyphasic approach. Strain CAU 9324(T)grew optimally at 30 degrees C and pH 6.0. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The major isoprenoid quinone was menaquinone-7 (MK-7). The polar lipid profile consisted of diphosphatidylglycerol as the major component. The predominant cellular fatty acid was anteiso-C-15:0. The DNA G + C content of strain CAU 9324(T)was 48.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the strain belonged to the genus Paenibacillus, showing <96.4% similarity to the type strains of all recognized Paenibacillus species. On the basis of phenotypic, chemotaxonomic and genotypic data, strain CAU 9324(T)was considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus puldeungensis sp. nov. is proposed. The type strain is CAU 9324(T) (=KCTC 13718(T) =CCUG 59189(T)).