Shikonin inhibits adipogenesis by modulation of the WNT/β-catenin pathway
DC Field | Value | Language |
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dc.contributor.author | Lee, Haeyong | - |
dc.contributor.author | Bae, Sungmin | - |
dc.contributor.author | Kim, Kijeong | - |
dc.contributor.author | Kim, Wonyong | - |
dc.contributor.author | Chung, Sang-In | - |
dc.contributor.author | Yang, Young | - |
dc.contributor.author | Yoon, Yoosik | - |
dc.date.available | 2019-05-29T13:40:15Z | - |
dc.date.issued | 2011-02 | - |
dc.identifier.issn | 0024-3205 | - |
dc.identifier.issn | 1879-0631 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21735 | - |
dc.description.abstract | Aim: Our previous study showed for the first time that shikonin, a natural compound isolated from Lithospermun erythrorhizon Sieb. Et Zucc, inhibits adipogenesis and fat accumulation. This study was conducted to investigate the molecular mechanism of the anti-adipogenic effects of shikonin. Main methods: Gene knockdown experiments using small interfering RNA (siRNA) transfection were conducted to elucidate the crucial role of beta-catenin in the anti-adipogenic effects of shikonin. Key findings: Shikonin prevented the down-regulation of beta-catenin and increased the level of its transcriptional product, cyclin D1, during adipogenesis of 3T3-L1 cells, preadipocytes originally derived from mouse embryo. beta-catenin was a crucial mediator of the anti-adipogenic effects of shikonin, as determined by siRNA-mediated knockdown. Shikonin-induced reductions of the major transcription factors of adipogenesis including peroxisome proliferator-activated receptor gamma and CCAAT/enhancer binding protein alpha, and lipid metabolizing enzymes including fatty acid binding protein 4 and lipoprotein lipase, as well as intracellular fat accumulation, were all significantly recovered by siRNA-mediated knockdown of beta-catenin. Among the genes located in the WNT/beta-catenin pathway, the levels of WNT10B and DVL2 were significantly up-regulated, whereas the level of AXIN was down-regulated by shikonin treatment. Significance: This study clearly shows that shikonin inhibits adipogenesis by the modulation of WNT/beta-catenin pathway in vitro, and also suggests that WNT/beta-catenin pathway can be used as a therapeutic target for obesity and related diseases using a natural compound like shikonin, even though the in vivo effects of shikonin and its clinical significance remain to be elucidated. (C) 2010 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Shikonin inhibits adipogenesis by modulation of the WNT/β-catenin pathway | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.lfs.2010.12.004 | - |
dc.identifier.bibliographicCitation | LIFE SCIENCES, v.88, no.7-8, pp 294 - 301 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000287224300002 | - |
dc.identifier.scopusid | 2-s2.0-79751536503 | - |
dc.citation.endPage | 301 | - |
dc.citation.number | 7-8 | - |
dc.citation.startPage | 294 | - |
dc.citation.title | LIFE SCIENCES | - |
dc.citation.volume | 88 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Shikonin | - |
dc.subject.keywordAuthor | Adipogenesis | - |
dc.subject.keywordAuthor | WNT | - |
dc.subject.keywordAuthor | β-catenin | - |
dc.subject.keywordAuthor | 3T3-L1 | - |
dc.subject.keywordPlus | ACTIVATED RECEPTOR-GAMMA | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | 3T3-L1 CELLS | - |
dc.subject.keywordPlus | ADIPOCYTE DIFFERENTIATION | - |
dc.subject.keywordPlus | INSULIN-RESISTANCE | - |
dc.subject.keywordPlus | C/EBP-ALPHA | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | OBESITY | - |
dc.subject.keywordPlus | GENE | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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