Expression of human β-defensin-2 in the prostate
DC Field | Value | Language |
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dc.contributor.author | Kim, Hae Jong | - |
dc.contributor.author | Jung, Ja Rang | - |
dc.contributor.author | Kim, Hea Jin | - |
dc.contributor.author | Lee, Shin Young | - |
dc.contributor.author | Chang, In Ho | - |
dc.contributor.author | Lee, Tae Jin | - |
dc.contributor.author | Kim, Wonyong | - |
dc.contributor.author | Myung, Soon Chul | - |
dc.date.available | 2019-05-30T00:16:30Z | - |
dc.date.issued | 2011-01 | - |
dc.identifier.issn | 1464-4096 | - |
dc.identifier.issn | 1464-410X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21841 | - |
dc.description.abstract | We found the expression of human beta-defensin-2 (HBD-2) in the prostate for the first time and LPS, a gram negative bacterial component, upregulated HBD-2 in prostate epithelial cells. We are looking for other antimicrobial peptides expressed in the prostate besides human beta-defensin-2. Also, we are studying the relationship between antimicrobial peptides and the development or progression of prostate diseases. OBJECTIVE To investigate the expression and regulation of human beta-defensin-2 (HBD-2) in the prostate. PATIENTS AND METHODS Normal human prostate epithelial cell line (RWPE-1), human prostate cancer cell lines (DU-145, PC-3), and paraffin-embedded prostate tissue from patients with benign prostatic hyperplasia (BPH) were analysed by RT-PCR and immunohistochemical staining. HBD-2 expression was also analysed by RT-PCR and ELISA in RWPE-1 cells treated with lipopolysaccharide (LPS). Nuclear factor-kappa B (NF-kappa B) activation was assessed by I kappa B alpha immunoblotting and electrophoretic mobility shift assay (EMSA). RESULTS BPH tissue and all of the tested prostate cell lines other than PC-3 constitutively express HBD-2 mRNA. HBD-2 protein was strongly detected in prostate gland tissue surrounded by inflammatory cells including macrophages. Exposure to LPS induced HBD-2 upregulation and NF-kappa B activation, as assessed by I kappa B alpha phosphorylation and degradation in RWPE-1 cells. Bay11-7082, an NF-kappa B inhibitor prevented LPS-induced HBD-2 production in RWPE-1 cells. CONCLUSIONS Prostate epithelial cells may constitutively express HBD-2, and its expression was upregulated by LPS. Our data indicate that HBD-2 may be an important immunomodulatory factor in prostate function. Expression of HBD-2 in normal prostates and the potential role of HBD-2 in prostatitis and BPH should be addressed in the future. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | Expression of human β-defensin-2 in the prostate | - |
dc.type | Article | - |
dc.identifier.doi | 10.1111/j.1464-410X.2010.09469.x | - |
dc.identifier.bibliographicCitation | BJU INTERNATIONAL, v.107, no.1, pp 144 - 149 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000285418400019 | - |
dc.identifier.scopusid | 2-s2.0-78650650219 | - |
dc.citation.endPage | 149 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 144 | - |
dc.citation.title | BJU INTERNATIONAL | - |
dc.citation.volume | 107 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | prostate | - |
dc.subject.keywordAuthor | human beta-defensin-2 | - |
dc.subject.keywordAuthor | lipopolysaccharide | - |
dc.subject.keywordAuthor | NF-kappa B activation | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | INTESTINAL EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | HUMAN-BETA DEFENSIN-2 | - |
dc.subject.keywordPlus | ANTIMICROBIAL PEPTIDES | - |
dc.subject.keywordPlus | INNATE IMMUNITY | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | TISSUES | - |
dc.relation.journalResearchArea | Urology & Nephrology | - |
dc.relation.journalWebOfScienceCategory | Urology & Nephrology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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