Cloning and functional analysis of the GSH1/MET1 gene complementing cysteine and glutathione auxotrophy of the methylotrophic yeast Hansenula polymorpha
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ubiyvovk, V.M. | - |
dc.contributor.author | Blazhenko, O.V. | - |
dc.contributor.author | Zimmermann, M. | - |
dc.contributor.author | Sohn, M.J. | - |
dc.contributor.author | Kang, H.A. | - |
dc.date.available | 2019-05-30T00:21:46Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0201-8470 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21929 | - |
dc.description.abstract | The Hansenula polymorpha GSH1/MET1 gene was cloned by complementation of glutathione-dependent growth of H. polymorpha gsh1 mutant isolated previously as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) resistant and cadmiumion sensitive clone. The H. polymorpha GSH1 gene was capable of restoring cadmium ion resistance, MNNG sensitivity, normal glutathione level and cell proliferation on minimal media without addition of cysteine or glutathione, when introduced into the gsh1 mutant cells. It was shown that the H. polymorpha GSH1 gene has homology to the Saccharomyces cerevisiae MET1 gene encoding S-adenosyl-L- methionine uroporphyrinogen III transmethylase, responsible for the biosynthesis of sulfite reductase cofactor, sirohaem. The H. polymorpha GSH1/MET1 gene deletion cassette (Hpgsh1/met1::ScLEU2) was constructed and corresponding null mutants were isolated. Crossing data of the point gsh1 and null gsh1/met1 mutants demonstrated that both alleles were located to the same gene. The null gsh1/met1 mutant showed total growth restoration on minimal media supplemented with cysteine or glutathione as a sole sulfur source, but not with inorganic (sulfate, sulfite) or organic (methionine, S-adenosylmethionine) sources of sulfur. Moreover, both the point gsh1 and null gsh1/met1 mutants displayed increased sensitivity to the toxic carbon substrate methanol, formaldehyde, organic peroxide and cadmiumions. | - |
dc.format.extent | 15 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.title | Cloning and functional analysis of the GSH1/MET1 gene complementing cysteine and glutathione auxotrophy of the methylotrophic yeast Hansenula polymorpha | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | Ukrain'skyi Biokhimichnyi Zhurnal, v.83, no.5, pp 67 - 81 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84856994283 | - |
dc.citation.endPage | 81 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 67 | - |
dc.citation.title | Ukrain'skyi Biokhimichnyi Zhurnal | - |
dc.citation.volume | 83 | - |
dc.identifier.url | http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?I21DBN=LINK&P21DBN=UJRN&Z21ID=&S21REF=10&S21CNR=20&S21STN=1&S21FMT=ASP_meta&C21COM=S&2_S21P03=FILA=&2_S21STR=BioChem_2011_83_5_11 | - |
dc.type.docType | Article | - |
dc.publisher.location | 우크라이나 | - |
dc.subject.keywordAuthor | Glutathione | - |
dc.subject.keywordAuthor | Hansenula polymorpha | - |
dc.subject.keywordAuthor | MET1 | - |
dc.subject.keywordAuthor | Methylotrophic yeast | - |
dc.subject.keywordAuthor | Sulfate assimilation | - |
dc.subject.keywordPlus | Pichia angusta | - |
dc.subject.keywordPlus | Saccharomyces cerevisiae | - |
dc.subject.keywordPlus | cadmium | - |
dc.subject.keywordPlus | cysteine | - |
dc.subject.keywordPlus | glutamate cysteine ligase | - |
dc.subject.keywordPlus | glutathione | - |
dc.subject.keywordPlus | GSH1 protein, S cerevisiae | - |
dc.subject.keywordPlus | methanol | - |
dc.subject.keywordPlus | methylnitronitrosoguanidine | - |
dc.subject.keywordPlus | methyltransferase | - |
dc.subject.keywordPlus | s adenosylmethionine | - |
dc.subject.keywordPlus | Saccharomyces cerevisiae protein | - |
dc.subject.keywordPlus | sulfate | - |
dc.subject.keywordPlus | uroporphyrin III C methyltransferase | - |
dc.subject.keywordPlus | uroporphyrin-III C-methyltransferase | - |
dc.subject.keywordPlus | amino acid sequence | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | chemistry | - |
dc.subject.keywordPlus | drug effect | - |
dc.subject.keywordPlus | enzymology | - |
dc.subject.keywordPlus | gene deletion | - |
dc.subject.keywordPlus | gene expression | - |
dc.subject.keywordPlus | genetic complementation | - |
dc.subject.keywordPlus | genetics | - |
dc.subject.keywordPlus | metabolism | - |
dc.subject.keywordPlus | methodology | - |
dc.subject.keywordPlus | molecular cloning | - |
dc.subject.keywordPlus | molecular genetics | - |
dc.subject.keywordPlus | Pichia | - |
dc.subject.keywordPlus | proteomics | - |
dc.subject.keywordPlus | Saccharomyces cerevisiae | - |
dc.subject.keywordPlus | sequence alignment | - |
dc.subject.keywordPlus | sequence homology | - |
dc.subject.keywordPlus | Amino Acid Sequence | - |
dc.subject.keywordPlus | Cadmium | - |
dc.subject.keywordPlus | Cloning, Molecular | - |
dc.subject.keywordPlus | Cysteine | - |
dc.subject.keywordPlus | Gene Deletion | - |
dc.subject.keywordPlus | Gene Expression | - |
dc.subject.keywordPlus | Genetic Complementation Test | - |
dc.subject.keywordPlus | Glutamate-Cysteine Ligase | - |
dc.subject.keywordPlus | Glutathione | - |
dc.subject.keywordPlus | Methanol | - |
dc.subject.keywordPlus | Methylnitronitrosoguanidine | - |
dc.subject.keywordPlus | Methyltransferases | - |
dc.subject.keywordPlus | Molecular Sequence Data | - |
dc.subject.keywordPlus | Pichia | - |
dc.subject.keywordPlus | Proteomics | - |
dc.subject.keywordPlus | S-Adenosylmethionine | - |
dc.subject.keywordPlus | Saccharomyces cerevisiae | - |
dc.subject.keywordPlus | Saccharomyces cerevisiae Proteins | - |
dc.subject.keywordPlus | Sequence Alignment | - |
dc.subject.keywordPlus | Sequence Homology, Amino Acid | - |
dc.subject.keywordPlus | Sulfates | - |
dc.subject.keywordPlus | Sulfuric Acid Esters | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.