Rapid identification and differentiation of Fasciola hepatica and Fasciola gigantica by a loop-mediated isothermal amplification (LAMP) assay
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ai, L. | - |
dc.contributor.author | Li, C. | - |
dc.contributor.author | Elsheikha, H. M. | - |
dc.contributor.author | Hong, S. J. | - |
dc.contributor.author | Chen, J. X. | - |
dc.contributor.author | Chen, S. H. | - |
dc.contributor.author | Li, X. | - |
dc.contributor.author | Cai, X. Q. | - |
dc.contributor.author | Chen, M. X. | - |
dc.contributor.author | Zhu, X. Q. | - |
dc.date.available | 2019-05-30T00:36:23Z | - |
dc.date.issued | 2010-12 | - |
dc.identifier.issn | 0304-4017 | - |
dc.identifier.issn | 1873-2550 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22009 | - |
dc.description.abstract | The present study developed and validated a species-specific loop-mediated isothermal amplification (LAMP) assay for the rapid detection and discrimination of Fasciola hepatica and Fasciola gigantica. The LAMP assay is inexpensive, easy to perform and shows rapid reaction, wherein the amplification can be obtained in 45 min under isothermal conditions of 61 degrees C or 62 degrees C by employing a set of four species-specific primer mixtures and results can be checked through naked-eye visualization. The optimal assay conditions with no cross-reaction with other closely related trematodes (Clonorchis sinensis, Opisthorchis viverrini, Orientobilharzia turkestanicum and Schistosoma japonicum) as well as within the two Fasciola species were established. The assay was validated by examining F. gigantica DNA in the intermediate host snails and in faecal samples. The results indicated that the LAMP assay is approximately 10(4) times more sensitive than the conventional specific PCR assays. These findings indicate that this Fasciola species-specific LAMP assay may have a potential clinical application for detection and differentiation of Fasciola species, especially in endemic countries. (C) 2010 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Rapid identification and differentiation of Fasciola hepatica and Fasciola gigantica by a loop-mediated isothermal amplification (LAMP) assay | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.vetpar.2010.09.005 | - |
dc.identifier.bibliographicCitation | VETERINARY PARASITOLOGY, v.174, no.3-4, pp 228 - 233 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000285232500007 | - |
dc.identifier.scopusid | 2-s2.0-78649332455 | - |
dc.citation.endPage | 233 | - |
dc.citation.number | 3-4 | - |
dc.citation.startPage | 228 | - |
dc.citation.title | VETERINARY PARASITOLOGY | - |
dc.citation.volume | 174 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Fasciola hepatica | - |
dc.subject.keywordAuthor | Fasciola gigantica | - |
dc.subject.keywordAuthor | Fascioliasis | - |
dc.subject.keywordAuthor | Loop-mediated isothermal amplification (LAMP) | - |
dc.subject.keywordAuthor | Rapid detection | - |
dc.subject.keywordAuthor | PCR | - |
dc.subject.keywordPlus | MOLECULAR CHARACTERIZATION | - |
dc.subject.keywordPlus | MITOCHONDRIAL-DNA | - |
dc.subject.keywordPlus | LYMNAEA-VIATRIX | - |
dc.subject.keywordPlus | TRIPLOID FORMS | - |
dc.subject.keywordPlus | RIBOSOMAL DNA | - |
dc.subject.keywordPlus | WATER SAMPLES | - |
dc.subject.keywordPlus | F-GIGANTICA | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | SEQUENCES | - |
dc.subject.keywordPlus | VIETNAM | - |
dc.relation.journalResearchArea | Parasitology | - |
dc.relation.journalResearchArea | Veterinary Sciences | - |
dc.relation.journalWebOfScienceCategory | Parasitology | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.