Delivery of shRNA using gold nanoparticle-DNA oligonucleotide conjugates as a universal carrier
DC Field | Value | Language |
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dc.contributor.author | Ryou, Sang-Mi | - |
dc.contributor.author | Kim, Sudeok | - |
dc.contributor.author | Jang, Hyun Hye | - |
dc.contributor.author | Kim, Jae-Hong | - |
dc.contributor.author | Yeom, Ji-Hyun | - |
dc.contributor.author | Eom, Min Sik | - |
dc.contributor.author | Bae, Jeehyeon | - |
dc.contributor.author | Han, Min Su | - |
dc.contributor.author | Lee, Kangseok | - |
dc.date.available | 2019-05-30T00:57:25Z | - |
dc.date.issued | 2010-07 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22320 | - |
dc.description.abstract | The efficient delivery of nucleic acids into mammalian cells is a central aspect of research involving cell biology and medical applications, including the clinical treatment of genetic disorders. We report an efficient small hairpin RNA (shRNA) delivery system that utilizes a single species of gold nanoparticle-DNA oligonucleotide conjugate (AuNP-DNA oligo) as a universal carrier. In vitro synthesized shRNA that is specific to the p53 gene was efficiently delivered into HEK293 and HeLa human cell lines using an AuNP-DNA oligo. The delivery resulted in an 80-90% knockdown of p53 expression. The same AuNP-DNA oligo was also efficient for the delivery of another shRNA, which is specific to the Mcl-1 gene, as well as the repression of MCL-1 expression. The knockdown efficiency of shRNA that was delivered using an AuNP-DNA oligo was comparable with that of a liposome-based shRNA delivery method. Our results offer an alternate delivery system for shRNA that can be used on any gene of interest. (C) 2010 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Delivery of shRNA using gold nanoparticle-DNA oligonucleotide conjugates as a universal carrier | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbrc.2010.06.115 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.398, no.3, pp 542 - 546 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000280867400036 | - |
dc.identifier.scopusid | 2-s2.0-77955279049 | - |
dc.citation.endPage | 546 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 542 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 398 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Gold nanoparticle | - |
dc.subject.keywordAuthor | Gene delivery system | - |
dc.subject.keywordAuthor | Gene knockdown | - |
dc.subject.keywordAuthor | shRNA | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | CONTROLLED-RELEASE | - |
dc.subject.keywordPlus | GENE-REGULATION | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | TRANSFECTION | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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