Brevibacillus fluminis sp nov., isolated from sediment of estuarine wetland

Abstract

Identification of a bacterial strain, designated CJ71(T), was carried out using a polyphasic taxonomic approach. Strain CJ71(T) was isolated from sediment from the estuarine wetland of the Han River, South Korea, by enrichment culture using pyrene as the sole carbon and energy source. The isolate was white-pigmented, rod-shaped, Gram-positive, strictly aerobic and motile. 16S rRNA gene sequence analysis revealed that strain CJ71(T) had the highest sequence similarity (96.9%) to Brevibacillus formosus DSM 9885(T). The predominant cellular fatty acids in strain CJ71(T) were anteiso-C-15:0 (49.5%), iso-C-15:0, (16.9%), iso-C-14:0 (16.9%) and iso-C-16:0 (4.9%). The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 52.4 mol%. Results from the polyphasic taxonomic study suggest that strain CJ71(T) represents a novel species of the genus Brevibacillus for which the name Brevibacillus fluminis sp. nov. is proposed; the type strain is CJ71(T) (=KACC 13381(T)=JCM 15716(T))