PROTECTIVE EFFECTS OF PHENOLICS FROM JUJUBE (ZIZIPHUS JUJUBA) LEAF AGAINST H2O2-INDUCED OXIDATIVE STRESS IN NEURONAL PC-12 CELLS

Abstract

We aimed to determine the antioxidant and neuronal cell protective effects of jujube (Ziziphus jujuba) leaf solvent fractions. The butanol fraction from jujube showed potent antioxidant activity in each assay, and its ABTS radical scavenging effect, FRAP, and MDA inhibition increased in a dose-dependent manner. Following H2O2 treatment, intracellular ROS accumulation significantly reduced when butanol fraction from jujube leaf was present in the PC12 cell media. In the MTT cell viability assay, the butanol fraction showed a protective effect against H2O2-induced neurotoxicity and inhibited LDH release into the medium (7.13-43.89%). Total phenols of the three solvent fractions were 72.50 (chloroform), 297.18 (butanol), and 17.93 (water) mg/g GAE. The predominant phenolic compounds in jujube leaves are rutin (57.07 mg/100 g) and quercetin (9.27 mg/100 g). These data suggest that butanol fraction from jujube leaf including phenolics may be useful natural antioxidants to reduce the risk of neurodegenerative diseases including Alzheimer's disease.