Molecular interaction between HAX-1 and XIAP inhibits apoptosis
DC Field | Value | Language |
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dc.contributor.author | Kang, Young Ji | - |
dc.contributor.author | Jang, Mi | - |
dc.contributor.author | Park, Yun Kyung | - |
dc.contributor.author | Kang, Sunghyun | - |
dc.contributor.author | Bae, Kwang-Hee | - |
dc.contributor.author | Cho, Sayeon | - |
dc.contributor.author | Lee, Chong-Kil | - |
dc.contributor.author | Park, Byoung Chul | - |
dc.contributor.author | Chi, Seung-Wook | - |
dc.contributor.author | Park, Sung Goo | - |
dc.date.available | 2019-05-30T01:40:03Z | - |
dc.date.issued | 2010-03 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22562 | - |
dc.description.abstract | Caspase-3 is an important executor caspase that plays an essential role in apoptosis. Recently, HS1-associated protein X1 (HAX-1) was found to be a substrate of caspase-3. Although HAX-1 has serve multifunctional roles in cellular functions such as cell survival and calcium homeostasis, the detailed functional mechanism of HAX-1 remains still unclear. In this study, we performed proteomic experiments to identify the HAX-1 interactome. Through immunoprecipitation and 2D gel electrophoresis, we identified X-linked inhibitor of apoptosis protein (XIAP) as a novel HAX-1-interacting protein. By performing the GST pull-down assay, we defined the interaction domains in HAX-1 and XIAP, showing that HAX-1 binds to the BIR2 and BIR3 domains of XIAP whereas XIAP binds to the C-terminal domain of HAX-1. In addition, surface plasma resonance experiments showed that both BIR2 and BIR3 domains of XIAP bind to HAX-1 with affinity similar to that of full-length XIAP, indicating that either domain is necessary and sufficient for tight binding to HAX-1. Taken together with the observation that HAX-1 suppresses the polyubiquitination of XIAP, the cell viability assay results suggest that the formation of the HAX-1-XIAP complex inhibits apoptosis by enhancing the stability of XIAP against proteosomal degradation. (C) 2010 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Molecular interaction between HAX-1 and XIAP inhibits apoptosis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbrc.2010.02.084 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.393, no.4, pp 794 - 799 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000276053800043 | - |
dc.identifier.scopusid | 2-s2.0-77949489092 | - |
dc.citation.endPage | 799 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 794 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 393 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | HAX-1 | - |
dc.subject.keywordAuthor | XIAP | - |
dc.subject.keywordAuthor | Protein interaction | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordPlus | PROTEIN LIGASE ACTIVITY | - |
dc.subject.keywordPlus | X-LINKED INHIBITOR | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | MITOCHONDRIAL PROTEIN | - |
dc.subject.keywordPlus | NMR STRUCTURE | - |
dc.subject.keywordPlus | BIR DOMAIN | - |
dc.subject.keywordPlus | CASPASE-3 | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | SMAC/DIABLO | - |
dc.subject.keywordPlus | MUTAGENESIS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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