Paenibacillus aestuarii sp nov., isolated from an estuarine wetland

Abstract

A novel bacterial strain designated CJ25(T) was isolated from the estuarine wetland of the Han river in Korea. Identification of this strain was carried out on the basis of polyphasic taxonomy. The isolate was Gram-staining-positive, rod-shaped, non-pigmented and motile. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate was closely related to Paenibacillus chondroitinus DSM 5051(T) with 96.1% similarity. The predominant fatty acids were anteiso-C-15:0 (50.25%), iso-C-16:0 (18.54%) and iso-C-15:0 (10.00%). The major isoprenoid quinone was MK-7. The G+C content of genomic DNA was 50 mol%. According to physiological data and 16S rRNA gene sequence analysis, the isolate was discriminated from related members of the genus Paenibacillus. Therefore, strain CJ25(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus aestuarii sp. nov. is proposed. The type strain is CJ25(T) (=KACC 13125(T) = JCM 15521(T)).