Purification of neutral sphingomyelinase 2 from bovine brain and its calcium-dependent activation
DC Field | Value | Language |
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dc.contributor.author | Kim, Seok Kyun | - |
dc.contributor.author | Ahn, Kyong Hoon | - |
dc.contributor.author | Jeon, Hyung Jun | - |
dc.contributor.author | Lee, Dong Hoon | - |
dc.contributor.author | Jung, Sung Yun | - |
dc.contributor.author | Jung, Kwang Mook | - |
dc.contributor.author | Kim, Dae Kyong | - |
dc.date.available | 2019-05-30T01:42:48Z | - |
dc.date.issued | 2010-02 | - |
dc.identifier.issn | 0022-3042 | - |
dc.identifier.issn | 1471-4159 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22632 | - |
dc.description.abstract | Ceramide is produced by sphingomyelinase (SMase) and it plays a key role in cellular responses such as apoptosis. In this study, we report the purification and characterization of neutral SMase2 (nSMase2) from bovine brain tissue. Triton X-100 extracts of bovine brain membranes were purified in nine steps, including sequential chromatography. The specific activity of purified nSMase increased 8183-fold over the brain membrane fraction. Purified nSMase showed similarities to nSMase2, which had been purified and cloned previously. Interestingly, purified nSMase2 was Ca2+-dependent and could be activated by micromolar concentrations of Ca2+ under Mg2+-free conditions. Ceramide generation was dependent upon the calcium ionophore A23187 and was observed in nSMase2-over-expressing COS-7 cells. This generation was suppressed by GW4869, an nSMase2 inhibitor, but not to fumonisin B1, an inhibitor of the de novo ceramide synthesis pathway. The present study demonstrates the Ca2+-dependent activation of nSMase2. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | Purification of neutral sphingomyelinase 2 from bovine brain and its calcium-dependent activation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1111/j.1471-4159.2009.06527.x | - |
dc.identifier.bibliographicCitation | JOURNAL OF NEUROCHEMISTRY, v.112, no.4, pp 1088 - 1097 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000273822200022 | - |
dc.identifier.scopusid | 2-s2.0-75149116783 | - |
dc.citation.endPage | 1097 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1088 | - |
dc.citation.title | JOURNAL OF NEUROCHEMISTRY | - |
dc.citation.volume | 112 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | calcium | - |
dc.subject.keywordAuthor | ceramide | - |
dc.subject.keywordAuthor | characterization | - |
dc.subject.keywordAuthor | neutral sphingomyelinase 2 | - |
dc.subject.keywordAuthor | purification | - |
dc.subject.keywordPlus | PHOSPHOLIPASE A(2) | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | CERAMIDE | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | EXOCYTOSIS | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | CHANNELS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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