The Guanylyl Cyclase Activator YC-1 Directly Inhibits the Voltage-Dependent K+ Channels in Rabbit Coronary Arterial Smooth Muscle Cells
DC Field | Value | Language |
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dc.contributor.author | Park, Won Sun | - |
dc.contributor.author | Ko, Jae-Hong | - |
dc.contributor.author | Ko, Eun A. | - |
dc.contributor.author | Son, Youn Kyoung | - |
dc.contributor.author | Hong, Da Hye | - |
dc.contributor.author | Jung, In Duk | - |
dc.contributor.author | Park, Yeong-Min | - |
dc.contributor.author | Choi, Tae-Hoon | - |
dc.contributor.author | Kim, Nari | - |
dc.contributor.author | Han, Jin | - |
dc.date.available | 2019-05-30T02:23:35Z | - |
dc.date.issued | 2010-01 | - |
dc.identifier.issn | 1347-8613 | - |
dc.identifier.issn | 1347-8648 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22694 | - |
dc.description.abstract | We investigated the effects of YC-1, an activator of soluble guanylyl cyclase (sGC), on voltage-dependent K+ (Kv) channels in smooth muscle cells from freshly isolated rabbit coronary arteries by using the whole-cell patch clamp technique. YC-1 inhibited the Kv current in a dose-dependent fashion with an apparent K-d of 9.67 mu M. It accelerated the decay rate of Kv channel inactivation without altering the kinetics of current activation. The rate constants of association and dissociation for YC-1 were 0.36 +/- 0.01 mu M-1.s(-1) and 3.44 +/- 0.22 s(-1), respectively. YC-1 did not have a significant effect on the steady-state activation and inactivation curves. The recovery time constant from inactivation was decreased in the presence of YC-1, and application of train pulses (1 or 2 Hz) caused a progressive increase in the YC-1 blockade, indicating that YC-1-induced inhibition of Kv currents is use-dependent. Pretreatment with Bay 41-2272 (also a sGC activator), ODQ (a sGC inhibitor), or Rp-8-Br-PET-cGMPs (a protein kinase G inhibitor) did not affect the basal Kv current and also did not significantly alter the inhibitory effect of YC-1. From these results, we suggest that YC-1 directly inhibits the Kv current independently of sGC activation and in a state-, time-, and use-dependent fashion. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | JAPANESE PHARMACOLOGICAL SOC | - |
dc.title | The Guanylyl Cyclase Activator YC-1 Directly Inhibits the Voltage-Dependent K+ Channels in Rabbit Coronary Arterial Smooth Muscle Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1254/jphs.09228FP | - |
dc.identifier.bibliographicCitation | JOURNAL OF PHARMACOLOGICAL SCIENCES, v.112, no.1, pp 64 - 72 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000273997000011 | - |
dc.identifier.scopusid | 2-s2.0-75749085400 | - |
dc.citation.endPage | 72 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 64 | - |
dc.citation.title | JOURNAL OF PHARMACOLOGICAL SCIENCES | - |
dc.citation.volume | 112 | - |
dc.type.docType | Article | - |
dc.publisher.location | 일본 | - |
dc.subject.keywordAuthor | YC-1 | - |
dc.subject.keywordAuthor | voltage-dependent K+ channel | - |
dc.subject.keywordAuthor | guanylyl cyclase | - |
dc.subject.keywordAuthor | coronary artery | - |
dc.subject.keywordPlus | PROTEIN-KINASE-A | - |
dc.subject.keywordPlus | HAMSTER OVARY CELLS | - |
dc.subject.keywordPlus | POTASSIUM CHANNELS | - |
dc.subject.keywordPlus | PHYSIOLOGICAL ROLES | - |
dc.subject.keywordPlus | KV1.3 CHANNELS | - |
dc.subject.keywordPlus | VASCULAR-TONE | - |
dc.subject.keywordPlus | ATP CHANNELS | - |
dc.subject.keywordPlus | DIRECT BLOCK | - |
dc.subject.keywordPlus | CURRENTS | - |
dc.subject.keywordPlus | MYOCYTES | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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