Induction of IL-8 expression by bacterial flagellin is mediated through lipid raft formation and intracellular TLR5 activation in A549 cells
DC Field | Value | Language |
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dc.contributor.author | Im, Jintaek | - |
dc.contributor.author | Jeon, Jun Ho | - |
dc.contributor.author | Cho, Min Kyung | - |
dc.contributor.author | Woo, Sang Su | - |
dc.contributor.author | Kang, Seok-Seong | - |
dc.contributor.author | Yun ,Cheol-Heui | - |
dc.contributor.author | Lee, Kangseok | - |
dc.contributor.author | Chung, Dae Kyun | - |
dc.contributor.author | Han, Seung Hyun | - |
dc.date.available | 2019-05-30T02:37:14Z | - |
dc.date.issued | 2009-12 | - |
dc.identifier.issn | 0161-5890 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22859 | - |
dc.description.abstract | We investigated the mechanism for the induction of a chemokine, IL-8, by bacterial flagellins in the human alveolar type 11 epithelial cell line, A549. Bacterial flagellin induced expression of IL-8 mRNA and protein in dose- and time-dependent manners. IL-8 expression was inhibited by nystatin (a lipid rafts inhibitor) but not by chlorpromazine (a clathrin-coated pits inhibitor). Interestingly, Toll-like receptor 5 (TLR5) recognizing flagellins was found in the intracellular compartment of A549 but rarely on the cell surface. Flagellin-induced IL-8 expression appears to be mediated through TLR5 as determined by in vitro transient transfection experiment in HEK-293 cells expressing TLR5 using a reporter gene construct containing IL-8 promoter. IL-8 expression was attenuated by inhibitors for protein kinase C (PKC) and mitogen-activated protein (MAP) kinases. Furthermore, NF-kappa B and NF-IL6 transcription factors played an important role in the flagellin-induced IL-8 gene expression in A549 cells. Collectively, these results suggest that flagellin-induced IL-8 expression requires formation of lipid rafts, intracellular TLR activation. and subsequent activation of PKC and MAP kinases leading to the activation of the transcription factors NF-kappa B and NF-IL6 in human alveolar type II epithelial cells. (C) 2009 Elsevier Ltd. All rights reserved. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Induction of IL-8 expression by bacterial flagellin is mediated through lipid raft formation and intracellular TLR5 activation in A549 cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.molimm.2009.09.004 | - |
dc.identifier.bibliographicCitation | MOLECULAR IMMUNOLOGY, v.47, no.2-3, pp 614 - 622 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000273103700055 | - |
dc.identifier.scopusid | 2-s2.0-70450245112 | - |
dc.citation.endPage | 622 | - |
dc.citation.number | 2-3 | - |
dc.citation.startPage | 614 | - |
dc.citation.title | MOLECULAR IMMUNOLOGY | - |
dc.citation.volume | 47 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Flagellin | - |
dc.subject.keywordAuthor | IL-8 | - |
dc.subject.keywordAuthor | Toll-like receptor 5 | - |
dc.subject.keywordAuthor | Lipid rafts | - |
dc.subject.keywordAuthor | A549 | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | INTERLEUKIN-8 GENE-EXPRESSION | - |
dc.subject.keywordPlus | PULMONARY EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | INNATE IMMUNE-RESPONSE | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | LIPOTEICHOIC ACID | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | KINASE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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