Identification of the large-conductance background K+ channel in mouse B cells as TREK-2
DC Field | Value | Language |
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dc.contributor.author | Zheng, Haifeng | - |
dc.contributor.author | Nam, Joo Hyun | - |
dc.contributor.author | Pang, Bo | - |
dc.contributor.author | Shin, Dong Hoon | - |
dc.contributor.author | Kim, Ji Seon | - |
dc.contributor.author | Chun, Yang-Sook | - |
dc.contributor.author | Park, Jong-Wan | - |
dc.contributor.author | Bang, Hyowon | - |
dc.contributor.author | Kim, Woo Kyung | - |
dc.contributor.author | Earm, Yung E. | - |
dc.contributor.author | Kim, Sung Joon | - |
dc.date.available | 2019-05-30T02:57:46Z | - |
dc.date.issued | 2009-07 | - |
dc.identifier.issn | 0363-6143 | - |
dc.identifier.issn | 1522-1563 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23129 | - |
dc.description.abstract | Zheng H, Nam JH, Pang B, Shin DH, Kim JS, Chun YS, Park JW, Bang H, Kim WK, Earm YE, Kim SJ. Identification of the large-conductance background K+ channel in mouse B cells as TREK-2. Am J Physiol Cell Physiol 297: C188-C197, 2009. First published May 13, 2009; doi:10.1152/ajpcell.00052.2009.-Mouse B cells and their cell line (WEHI-231) express large-conductance background K+ channels (LKbg) that are activated by arachidonic acids, characteristics similar to TREK-2. However, there is no evidence to identify the molecular nature of LKbg; some properties of LKbg were partly different from the reported results of TREK type channels. In this study, we compared the properties of cloned TREK-2 and LKbg in terms of their sensitivities to ATP, phosphatidylinositol 4,5-bisphosphate (PIP2), intracellular pH (pH(i)), and membrane stretch. Similar to the previous findings of LKbg, TREK-2 showed spontaneous activation after membrane excision (i-o patch) and were inhibited by MgATP or by PIP2. The inhibition by MgATP was prevented by wortmannin, suggesting membrane-delimited regulation of TREKs by phosphoinositide (PI) kinase. The same was observed with the property of LKbg; the activation of TREK-2 by membrane stretch was suppressed by U73122 (PLC inhibitor). As with the known properties of TREK-2, LKbg were activated by acidic pH(i) and inhibited by PKC activator. Finally, we confirmed the expression of TREK-2 in WEHI-231 by using RT-PCR and immunoblot analyses. The amplitude of background K+ current and the TREK-2 expression in WEHI-231 were commonly decreased by genetic knockdown of TREK-2 using small interfering RNA. The downregulation of TREK-2 attenuated Ca2+-influx induced by arachidonic acid in WEHI-231. As a whole, these results strongly indicate that TREK-2 encodes LKbg in mouse B cells. We also newly suggest that the low activity of TREK-2 in intact cells is due to the inhibition by intrinsic PIP2. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER PHYSIOLOGICAL SOC | - |
dc.title | Identification of the large-conductance background K+ channel in mouse B cells as TREK-2 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1152/ajpcell.00052.2009 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, v.297, no.1, pp C188 - C197 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000268497000021 | - |
dc.identifier.scopusid | 2-s2.0-67650071218 | - |
dc.citation.endPage | C197 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | C188 | - |
dc.citation.title | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | - |
dc.citation.volume | 297 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | K2P channel | - |
dc.subject.keywordAuthor | arachidonic acid | - |
dc.subject.keywordAuthor | PI kinase | - |
dc.subject.keywordAuthor | membrane stretch | - |
dc.subject.keywordAuthor | immune cells | - |
dc.subject.keywordPlus | ION CHANNELS | - |
dc.subject.keywordPlus | FUNCTIONAL-CHARACTERISTICS | - |
dc.subject.keywordPlus | POTASSIUM CHANNELS | - |
dc.subject.keywordPlus | FATTY-ACIDS | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | PIP2 | - |
dc.subject.keywordPlus | IMMUNOMODULATION | - |
dc.subject.keywordPlus | LYMPHOCYTES | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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