Crystallization and preliminary X-ray crystallographic analysis of Escherichia coli CusB
DC Field | Value | Language |
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dc.contributor.author | Xu, Yongbin | - |
dc.contributor.author | Yun, Bo-Young | - |
dc.contributor.author | Sim, Se-Hoon | - |
dc.contributor.author | Lee, Kangseok | - |
dc.contributor.author | Ha, Nam-Chul | - |
dc.date.available | 2019-05-30T02:57:50Z | - |
dc.date.issued | 2009-07 | - |
dc.identifier.issn | 1744-3091 | - |
dc.identifier.issn | 2053-230X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23131 | - |
dc.description.abstract | Periplasmic membrane-fusion proteins (MFPs) are an essential component of multidrug and metal-efflux pumps in Gram-negative bacteria. However, the functional structure of MFPs remains unclear. CusCFBA, the Cu-I and Ag-I efflux system in Escherichia coli, consists of the MFP CusB, the OMF CusC and the RND-type transporter CusA. The MFP CusB bridges the inner membrane RND-type efflux transporter CusA and the outer membrane factor CusC and exhibits substrate-linked conformational changes which distinguish it from other MFP-family members. CusB from E. coli was overexpressed and the recombinant protein was purified using Ni-NTA affinity, Q anion-exchange and gel-filtration chromatography. The purified CusB protein was crystallized using the vapour-diffusion method. A diffraction data set was collected to a resolution of 3.1 angstrom at 100 K. The crystal belonged to space group C222. | - |
dc.format.extent | 3 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | International Union of Crystallography | - |
dc.title | Crystallization and preliminary X-ray crystallographic analysis of Escherichia coli CusB | - |
dc.type | Article | - |
dc.identifier.doi | 10.1107/S1744309109019873 | - |
dc.identifier.bibliographicCitation | Acta Crystallographica Section F: Structural Biology and Crystallization Communications, v.65, no.7, pp 743 - 745 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000267530000024 | - |
dc.identifier.scopusid | 2-s2.0-68149106026 | - |
dc.citation.endPage | 745 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 743 | - |
dc.citation.title | Acta Crystallographica Section F: Structural Biology and Crystallization Communications | - |
dc.citation.volume | 65 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Gram-negative bacteria | - |
dc.subject.keywordAuthor | Membrane-fusion proteins | - |
dc.subject.keywordAuthor | Metal-efflux pumps | - |
dc.subject.keywordAuthor | RND-type transporters | - |
dc.subject.keywordPlus | EFFLUX PUMP | - |
dc.subject.keywordPlus | PERIPLASMIC COMPONENT | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | MULTIDRUG EFFLUX | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | TRANSPORTER | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | ACRA | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Crystallography | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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