Mutation in the DNA-binding domain of the EWS-Oct-4 oncogene results in dominant negative activity that interferes with EWS-Oct-4-mediated transactivation
DC Field | Value | Language |
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dc.contributor.author | Kim, Sol | - |
dc.contributor.author | Lee, Jungwoon | - |
dc.contributor.author | Kim, Ja Young | - |
dc.contributor.author | Lim, Bobae | - |
dc.contributor.author | Shin, Eung-Kyun | - |
dc.contributor.author | Han, Yong-Mahn | - |
dc.contributor.author | Kim, Sung-Su | - |
dc.contributor.author | Song, Jin-Ho | - |
dc.contributor.author | Kirn, Jungho | - |
dc.date.available | 2019-05-30T03:33:25Z | - |
dc.date.issued | 2009-05 | - |
dc.identifier.issn | 0020-7136 | - |
dc.identifier.issn | 1097-0215 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23182 | - |
dc.description.abstract | The EWS-Oct-4 protein is a chimeric molecule in which the amino terminal domain (NTD) of the EWS becomes fused to the carboxy terminal domain (CTD) of the Cict-4 transcription factor. It was identified in human bone and soft-tissue tumors associated with t(6;22)(p21;q12). Using in vitro and in vivo systems, we found that the EWS-Oct-4 protein self-associates. The major domains required for self-association mapped to the EWS NTD (amino acids 70-163) and the POU DNA-binding domain. EWS-Oct-4 protein also associated with EWS-Oct-4 (V351P), which contains a mutation in the POU DNA-binding domain. Using electrophoretic mobility shift assays, we found that the EWS-Oct-4 (V351P) mutant interfered with wild-type EWS-Oct-4 DNA-binding activity. In addition, we found that EWS-Oct-4-mediated transcriptional activation was inhibited by EWS-Oct-4 (V351P) protein in vivo. Thus, this mutation in the POU DNA-binding domain results in a dominant negative protein. These findings suggest that the biological functions of the EWS-Oct-4 oncogene can be modulated by the dominant negative mutant EWS-Oct-4 (V351P). (C) 2008 Wiley-Liss, Inc. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | Mutation in the DNA-binding domain of the EWS-Oct-4 oncogene results in dominant negative activity that interferes with EWS-Oct-4-mediated transactivation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/ijc.24228 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF CANCER, v.124, no.10, pp 2312 - 2322 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000265353200007 | - |
dc.identifier.scopusid | 2-s2.0-64249141070 | - |
dc.citation.endPage | 2322 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 2312 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF CANCER | - |
dc.citation.volume | 124 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | EWS-Oct-4 | - |
dc.subject.keywordAuthor | self-association | - |
dc.subject.keywordAuthor | dominant negative | - |
dc.subject.keywordAuthor | chromosomal translocation | - |
dc.subject.keywordAuthor | transactivation | - |
dc.subject.keywordAuthor | transformation | - |
dc.subject.keywordAuthor | bone and soft-tissue tumors | - |
dc.subject.keywordPlus | GERM-CELL TUMORS | - |
dc.subject.keywordPlus | EWINGS-SARCOMA PROTEIN | - |
dc.subject.keywordPlus | BREAST-CANCER CELLS | - |
dc.subject.keywordPlus | RNA-POLYMERASE-II | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | EMBRYONIC GENES | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | POU-DOMAIN | - |
dc.subject.keywordPlus | OCT-4 | - |
dc.subject.keywordPlus | EWS | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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