Effects of Escherichia coli RraA Orthologs of Vibrio vulnificus on the Ribonucleolytic Activity of RNase E In Vivo
DC Field | Value | Language |
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dc.contributor.author | Lee ,Minho | - |
dc.contributor.author | Yeom, Ji-Hyun | - |
dc.contributor.author | Sim, Se-Hoon | - |
dc.contributor.author | Ahn, Sangmi | - |
dc.contributor.author | Lee, Kangseok | - |
dc.date.available | 2019-05-30T03:36:17Z | - |
dc.date.issued | 2009-04 | - |
dc.identifier.issn | 0343-8651 | - |
dc.identifier.issn | 1432-0991 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23265 | - |
dc.description.abstract | RraA is a recently discovered protein inhibitor of RNase E that catalyzes the initial step in the decay and processing of numerous RNAs in Escherichia coli. In the genome of Vibrio vulnificus, two open reading frames that potentially encode proteins homologous to E. coli, RraA-designated RraAV1 and RraAV2, have respectively 80.1% and 59.0% amino acid identity to RraA. The authors report that coexpression of RraAV1 protein in E. coli cells overproducing RNase E rescued these cells from growth arrest and restored their normal growth, whereas coexpression of RraAV2 protein further inhibited the growth of E. coli cells, whose growth was already impaired by overproduction of RNase E. Analyses of the steady-state level of various RNase E substrates indicated that the coexpression of RraAV1 more efficiently inhibited RNase E action than coexpression of RraA, and consequently resulted in the more increased abundance of each RNA species tested in vivo. The inhibitory effect by RraAV2 coexpression on RNase E was observed only in the case of trpA mRNA, indicating the possibility of RNA substrate-dependent inhibition of RraAV2 on RNase E. The findings suggest that these regulators of ribonuclease activity have both a conserved inhibitory function and a differential inhibitory activity on RNase E-like enzymes across the species. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER | - |
dc.title | Effects of Escherichia coli RraA Orthologs of Vibrio vulnificus on the Ribonucleolytic Activity of RNase E In Vivo | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s00284-009-9360-8 | - |
dc.identifier.bibliographicCitation | CURRENT MICROBIOLOGY, v.58, no.4, pp 349 - 353 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000263869200012 | - |
dc.identifier.scopusid | 2-s2.0-65549170705 | - |
dc.citation.endPage | 353 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 349 | - |
dc.citation.title | CURRENT MICROBIOLOGY | - |
dc.citation.volume | 58 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | DEGRADOSOME | - |
dc.subject.keywordPlus | ABUNDANCE | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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