LRRC3B, encoding a leucine-rich repeat-containing protein, is a putative tumor suppressor gene in gastric cancer
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Mirang | - |
dc.contributor.author | Kim, Jeong-Hwan | - |
dc.contributor.author | Jang, Hay-Ran | - |
dc.contributor.author | Kim, Hwan-Mook | - |
dc.contributor.author | Lee, Chang-Woo | - |
dc.contributor.author | Noh, Seung-Moo | - |
dc.contributor.author | Song, Kyu-Sang | - |
dc.contributor.author | Cho, June-Sik | - |
dc.contributor.author | Jeong, Hyun-Yong | - |
dc.contributor.author | Hahn, Yoonsoo | - |
dc.contributor.author | Yeom, Young-Il | - |
dc.contributor.author | Yoo, Hyang-Sook | - |
dc.contributor.author | Kim, Yong Sung | - |
dc.date.available | 2019-05-30T04:38:51Z | - |
dc.date.issued | 2008-09 | - |
dc.identifier.issn | 0008-5472 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23625 | - |
dc.description.abstract | Leucine-rich repeat-containing 3B (LRRC3B) is an evolutionarily highly conserved leucine-rich repeat-containing protein, but its biological significance is unknown. Using restriction landmark genomic scanning and pyrosequencing, we found that the promoter region of LRRC3B was aberrantly methylated in gastric cancer. Gastric cancer cell lines displayed epigenetic silencing of LRRC3B, but treatment with the DNA methylation inhibitor 5-aza-2'-deoxycytidine and/or the histone deacetylase inhibitor trichostatin A increased LRRC3B expression in gastric cancer cell lines. Real-time reverse transcription-PCR analysis of 96 paired primary gastric tumors and normal adjacent tissues showed that LRRC3B expression was reduced in 88.5% of gastric tumors compared with normal adjacent tissues. Pyrosequencing analysis of the promoter region revealed that LRRC3B was significantly hypermethylated in gastric tumors. Stable transfection of LRRC3B in SNU-601 cells, a gastric cancer cell line, inhibited anchorage-dependent and anchorage-independent colony formation, and LRRC3B expression suppressed tumorigenesis in nude mice. Microarray analysis of LRRC3B-expressing xenograft tumors showed induction of immune response-related genes and IFN signaling genes. H&E-stained sections of LRRC3B-expressing xenograft tumors showed lymphocyte infiltration in the region. We suggest that LRRC3B is a putative tumor suppressor gene that is silenced in gastric cancers by epigenetic mechanisms and that LRRC3B silencing in cancer may play an important role in tumor escape front immune surveillance. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER ASSOC CANCER RESEARCH | - |
dc.title | LRRC3B, encoding a leucine-rich repeat-containing protein, is a putative tumor suppressor gene in gastric cancer | - |
dc.type | Article | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-08-0667 | - |
dc.identifier.bibliographicCitation | CANCER RESEARCH, v.68, no.17, pp 7147 - 7155 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000259080300036 | - |
dc.identifier.scopusid | 2-s2.0-52049115152 | - |
dc.citation.endPage | 7155 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 7147 | - |
dc.citation.title | CANCER RESEARCH | - |
dc.citation.volume | 68 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | 2-DIMENSIONAL GEL ANALYSIS | - |
dc.subject.keywordPlus | CELL-LINES | - |
dc.subject.keywordPlus | EPIGENETIC INACTIVATION | - |
dc.subject.keywordPlus | DNA METHYLATION | - |
dc.subject.keywordPlus | CDNA MICROARRAY | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | GENOMIC DNA | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.