Fluorescent labeling of cell-free synthesized proteins with fluorophore-conjugated methionylated tRNA derived from in vitro transcribed tRNA
DC Field | Value | Language |
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dc.contributor.author | Jun, Soo Youn | - |
dc.contributor.author | Kang, Sang Hyeon | - |
dc.contributor.author | Lee, Kwang-Ho | - |
dc.date.available | 2019-05-30T04:42:49Z | - |
dc.date.issued | 2008-06 | - |
dc.identifier.issn | 0167-7012 | - |
dc.identifier.issn | 1872-8359 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23727 | - |
dc.description.abstract | A simple and practical method for preparing fluorophore-conjugated methionylated tRNA necessary for tRNA-mediated fluorescent labeling of cell-free synthesized proteins was developed. Without complicated chromatographic purification and subsequent concentration, fluorophore-conjugated methionylated tRNA with higher purity and fluorescence concentration could be synthesized from in vitro transcribed tRNA instead of from a total IRNA mixture, which has been routinely used as a tRNA source. Although fluorophore-conjugated methionylated tRNA derived from in vitro transcribed tRNA was purified by simple phenol extraction following alcohol precipitation, it worked well in tRNA-mediated fluorescent labeling, yielding an improved signal-to-noise ratio and higher fluorescence intensity compared to the conventional total tRNA-based method. Based on its simplicity in the preparation of labeling agent with higher purity and fluorescence concentration, the developed method will accelerate the prevalence of fluorescence-based detection of cell-free synthesized proteins. (C) 2008 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Fluorescent labeling of cell-free synthesized proteins with fluorophore-conjugated methionylated tRNA derived from in vitro transcribed tRNA | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.mimet.2008.02.016 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGICAL METHODS, v.73, no.3, pp 247 - 251 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000256271200006 | - |
dc.identifier.scopusid | 2-s2.0-43049178190 | - |
dc.citation.endPage | 251 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 247 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGICAL METHODS | - |
dc.citation.volume | 73 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | cell-free synthesized proteins | - |
dc.subject.keywordAuthor | fluorescence | - |
dc.subject.keywordAuthor | labeling | - |
dc.subject.keywordAuthor | in vitro transcription | - |
dc.subject.keywordPlus | NONNATURAL AMINO-ACIDS | - |
dc.subject.keywordPlus | SYNTHESIS SYSTEM | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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