Sulfamethazine detection with direct-binding optical waveguide lightmode spectroscopy-based immunosensor
DC Field | Value | Language |
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dc.contributor.author | Kim, Namsoo | - |
dc.contributor.author | Kim, Dong-Kyung | - |
dc.contributor.author | Kim, Woo-Yeon | - |
dc.date.available | 2019-05-30T05:32:18Z | - |
dc.date.issued | 2008-05 | - |
dc.identifier.issn | 0308-8146 | - |
dc.identifier.issn | 1873-7072 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23750 | - |
dc.description.abstract | A direct-binding optical waveguide lightmode spectroscopy-based immunosensor detecting sulfamethazine (SMZ) was prepared, followed by the measurement of its specificity and sensitivity. System construction was undertaken with a peristaltic pump, an injector and the main unit comprising a sensor holder, two signal-harvesting photodiodes, a beam mirror, shutter and He-Ne laser source emitting a monochrome light (lambda = 632.8 nm), plus a PC. Antibody immobilization was performed in situ by covalent binding of an anti-SMZ antibody over the surface of a glutaraldehyde-activated 3-aminopropyltriethoxysitane-treated sensor chip. The reaction buffer for the system was 4 mM Tris-HCl (pH 7.2) that showed a medium surface coverage and stable baseline. Sensor response was quite specific to antibody-antigen complexation, as judged from no sensor response caused by bovine serum albumin immobilization. The sensor responses according to SMZ concentrations from 10(-8) to 10(-2) M increased linearly in a semi-logarithmic scale, with the limit of detection of 10(-8) M. The immunosensor was favorably reusable for SMZ screening. (C) 2007 Elsevier Ltd. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Sulfamethazine detection with direct-binding optical waveguide lightmode spectroscopy-based immunosensor | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.foodchem.2007.11.018 | - |
dc.identifier.bibliographicCitation | FOOD CHEMISTRY, v.108, no.2, pp 768 - 773 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000253033400042 | - |
dc.identifier.scopusid | 2-s2.0-38049070308 | - |
dc.citation.endPage | 773 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 768 | - |
dc.citation.title | FOOD CHEMISTRY | - |
dc.citation.volume | 108 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | optical waveguide lightmode spectroscopy-based immunosensor | - |
dc.subject.keywordAuthor | sulfamethazine detection | - |
dc.subject.keywordAuthor | specificity and sensitivity | - |
dc.subject.keywordPlus | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | SURFACE-PLASMON RESONANCE | - |
dc.subject.keywordPlus | FLUORESCENCE DETECTION | - |
dc.subject.keywordPlus | GRATING COUPLER | - |
dc.subject.keywordPlus | POSTCOLUMN DERIVATIZATION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | BIOSENSOR ASSAY | - |
dc.subject.keywordPlus | EDIBLE TISSUES | - |
dc.subject.keywordPlus | RESIDUES | - |
dc.subject.keywordPlus | MILK | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Nutrition & Dietetics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Nutrition & Dietetics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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