Technical note: improved ancient DNA purification for PCR using ion-exchange columns
DC Field | Value | Language |
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dc.contributor.author | Kim, Kijeong | - |
dc.contributor.author | Kim, Kyung-Yong | - |
dc.contributor.author | Jeon, Eunhee | - |
dc.contributor.author | Togloom, Ariunaa | - |
dc.contributor.author | Cho, Youn-Ock | - |
dc.contributor.author | Lee, Min-Soo | - |
dc.contributor.author | Lkhagvasuren, Gavaachimed | - |
dc.contributor.author | Choi, Jee-Hye | - |
dc.contributor.author | Tumen, Dashtseveg | - |
dc.contributor.author | Park, Ae Ja | - |
dc.contributor.author | Kim, Keun-Cheol | - |
dc.contributor.author | Park, Ki-Won | - |
dc.contributor.author | Kim, Jae-Hyun | - |
dc.contributor.author | Noh, Maengseok | - |
dc.contributor.author | Yoo, Kwon-Jong | - |
dc.contributor.author | Lee, Kwang-Ho | - |
dc.date.available | 2019-05-30T05:33:06Z | - |
dc.date.issued | 2008-05 | - |
dc.identifier.issn | 0002-9483 | - |
dc.identifier.issn | 1096-8644 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23772 | - |
dc.description.abstract | A novel method of ancient DNA (aDNA) purification was developed using ion-exchange columns to improve PCR-amplifiable DNA extraction from ancient bone samples. Thirteen PCR-resistant ancient bone samples aged 500-3,300 years were tested to extract aDNA using a recently reported, silica-based aDNA extraction method and an ion-exchange column method for the further purification. The PCR success rates of the aDNA extracts were evaluated for the amplification ability of the fragments of mitochondrial DNA, a high-copy DNA, and amelogenin, a low-copy DNA. The results demonstrate that the further purification of silica-based aDNA extracts using ion-exchange columns considerably improved PCR amplification. We suggest that the ion-exchange column-based method will be useful for the improvement of PCR-amplifiable aDNA extraction, particularly from the poorly preserved, PCR-resistant, ancient samples. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | Technical note: improved ancient DNA purification for PCR using ion-exchange columns | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/ajpa.20782 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, v.136, no.1, pp 114 - 121 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000255067500012 | - |
dc.identifier.scopusid | 2-s2.0-42149168520 | - |
dc.citation.endPage | 121 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 114 | - |
dc.citation.title | AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY | - |
dc.citation.volume | 136 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | DNA purification | - |
dc.subject.keywordAuthor | ancient bone | - |
dc.subject.keywordAuthor | PCR amplification | - |
dc.subject.keywordAuthor | ion-exchange columns | - |
dc.subject.keywordAuthor | PCR inhibitors | - |
dc.subject.keywordPlus | MITOCHONDRIAL-DNA | - |
dc.subject.keywordPlus | HUMAN REMAINS | - |
dc.subject.keywordPlus | NUCLEAR-DNA | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | BONE | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | SPECIMENS | - |
dc.relation.journalResearchArea | Anthropology | - |
dc.relation.journalResearchArea | Evolutionary Biology | - |
dc.relation.journalWebOfScienceCategory | Anthropology | - |
dc.relation.journalWebOfScienceCategory | Evolutionary Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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