Ceramide induces p38 MAPK-dependent apoptosis and Bax translocation via inhibition of Akt in HL-60 cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Hae Jong | - |
dc.contributor.author | Oh, Ji Eun | - |
dc.contributor.author | Kim, Sang Woo | - |
dc.contributor.author | Chun, Young Jin | - |
dc.contributor.author | Kim, Mie Young | - |
dc.date.available | 2019-05-30T05:35:51Z | - |
dc.date.issued | 2008-02 | - |
dc.identifier.issn | 0304-3835 | - |
dc.identifier.issn | 1872-7980 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23842 | - |
dc.description.abstract | Ceramide induces apoptosis through caspase activation, cytochrome c release, and Bax translocation in HL-60 cells. However, the upstream signal transduction pathways that induce Bax translocation during ceramide-mediated apoptosis have not been well defined yet. In this study, the activation of p38 mitogen-activated protein kinase (MAPK) was found to be critical for the induction of apoptosis and subcellular redistribution of Bax. Pharmacological inhibition of p38 MAPK with SB203580 or expression of a dominant-negative p38 MAPK attenuated DNA fragmentation, caspase-3 activation, and Bax translocation in response to ceramide. Overexpression of Akt also led to suppression of Bax translocation to mitochondria during ceramide-induced apoptosis in HL-60 cells. We also provide evidence for cross-talk between p38 MAPK and Akt pathways. Expression of myr-Akt or inhibition of phosphatidylinositol 3-kinase (PI3K) with LY294002 had no effect on p38 MAPK activation by ceramide as assessed by phosphorylation, while inhibition of p38 MAPK by a pharmacological inhibitor or a dominant-negative p38 inhibited Akt dephosphorylation in response to ceramide, suggesting that ceramide-induced p38 MAPK activation negatively regulates the Akt pathway. (C) 2007 Elsevier Ireland Ltd. All rights reserved. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.title | Ceramide induces p38 MAPK-dependent apoptosis and Bax translocation via inhibition of Akt in HL-60 cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.canlet.2007.10.030 | - |
dc.identifier.bibliographicCitation | CANCER LETTERS, v.260, no.1-2, pp 88 - 95 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000253278400011 | - |
dc.identifier.scopusid | 2-s2.0-38149129507 | - |
dc.citation.endPage | 95 | - |
dc.citation.number | 1-2 | - |
dc.citation.startPage | 88 | - |
dc.citation.title | CANCER LETTERS | - |
dc.citation.volume | 260 | - |
dc.type.docType | Article | - |
dc.publisher.location | 아일랜드 | - |
dc.subject.keywordAuthor | ceramide | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | p38 MAPK | - |
dc.subject.keywordAuthor | Akt | - |
dc.subject.keywordAuthor | Bax | - |
dc.subject.keywordAuthor | HL-60 cells | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASES | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | CELLULAR-RESPONSES | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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