Purification and characterization of thermostable beta-glucosidase from the brown-rot basidiomycete Fomitopsis palustris grown on microcrystalline cellulose
DC Field | Value | Language |
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dc.contributor.author | Yoon, Jeong-Jun | - |
dc.contributor.author | Kim, Ki-Yeon | - |
dc.contributor.author | Cha, Chang-Jun | - |
dc.date.available | 2019-05-30T05:36:16Z | - |
dc.date.issued | 2008-02 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.issn | 1976-3794 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23853 | - |
dc.description.abstract | An extracellular beta-glucosidase was purified 154-fold to electrophoretic homogeneity from the brown-rot basidiomycete Fomitopsis palustris grown on 2.0% microcrystalline cellulose. SDS-polyacrylamide gel electrophoresis gel gave a single protein band and the molecular mass of purified enzyme was estimated to be approximately 138 kDa. The amino acid sequences of the proteolytic fragments determined by nano-LC-MS/MS suggested that the protein has high homology with fungal beta-glucosidases that belong to glycosyl hydrolase family 3. The K(m)s for p-nitorophenyl-beta-D-glucoside (p-NPG) and cellobiose hydrolyses were 0.117 and 4.81 mM, and the K-cat values were 721 and 101.8 per see, respectively. The enzyme was competitively inhibited by both glucose (K-i= 0.35 mM) and gluconolactone (K-i= 0.008 mM), when p-NPG was used as substrate. The optimal activity of the purified beta-glucosidase was observed at pH 4.5 and 70 degrees C. The F. palustris protein exhibited half-lives of 97 h at 55 degrees C and 15 h at 65 degrees C, indicating some degree of thermostability. The enzyme has high activity against p-NPG and cellobiose but has very little or no activity against p-nitrophenyl-beta-lactoside, p-nitrophenyl-beta-xyloside, p-nitrophenyl-alpha-arabinofuranoside, xylan, and carboxymethyl cellulose. Thus, our results revealed that the beta-glucosidase from F. palustris can be classified as an aryl-beta-glucosidase with cellobiase activity. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.title | Purification and characterization of thermostable beta-glucosidase from the brown-rot basidiomycete Fomitopsis palustris grown on microcrystalline cellulose | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s12275-007-0230-4 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.46, no.1, pp 51 - 55 | - |
dc.identifier.kciid | ART001247675 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000253545600009 | - |
dc.identifier.scopusid | 2-s2.0-43249094258 | - |
dc.citation.endPage | 55 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 51 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 46 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | beta-glucosidase | - |
dc.subject.keywordAuthor | brown-rot fungus | - |
dc.subject.keywordAuthor | Fomitopsis palustris | - |
dc.subject.keywordAuthor | purification | - |
dc.subject.keywordAuthor | microcrystalline cellulose | - |
dc.subject.keywordPlus | TRICHODERMA-REESEI | - |
dc.subject.keywordPlus | PHANEROCHAETE-CHRYSOSPORIUM | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | HYDROLYSIS | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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