Molecular cloning and enzymatic characterization of a class mu glutathione S-transferase of Paragonimus westermani
DC Field | Value | Language |
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dc.contributor.author | Kim, Tae Yun | - |
dc.contributor.author | Lee, Ji-Yun | - |
dc.contributor.author | Kim, Tae Im | - |
dc.contributor.author | Moon, Ki Ho | - |
dc.contributor.author | Kang, Shin-Yong | - |
dc.contributor.author | Hong, Sung-Jong | - |
dc.date.available | 2019-05-30T05:39:56Z | - |
dc.date.issued | 2007-10 | - |
dc.identifier.issn | 0932-0113 | - |
dc.identifier.issn | 1432-1955 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23953 | - |
dc.description.abstract | Glutathione S-transferase (GST) is a component of a second line of defense against bioreactive radicals derived from host immune attack. Paragonimus westermani causes acute or chronic lung diseases in mammals. A cDNA clone, PwGST#11, of adult P. westermani produced in the present study was 748 bp long and encoded an open reading frame of 217 amino acids with a starting methionine. The molecular mass of this putative polypeptide, Pw26GST, was estimated to be 25.1 kDa with an isoelectric point of 5.7. Pw26GST was homologous with the 26-kDa GSTs of trematodes and vertebrates. Nine of the ten amino acid residues lining the glutathione-binding pocket were conserved. Putative Pw26GST polypeptide was clustered with 26-kDa GSTs of trematodes belonging to the class mu. Recombinant Pw26GST protein generated bacterially, revealed GST enzyme activity toward an universal and class mu-specific substrates. Mouse antisera to recombinant Pw26GST protein recognized native 26-kDa GST of P. westermani but not the GSTs of any other trematodes. Collectively, Pw26GST was found to be a member of class mu GSTs of P. westermani. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER | - |
dc.title | Molecular cloning and enzymatic characterization of a class mu glutathione S-transferase of Paragonimus westermani | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s00436-007-0626-4 | - |
dc.identifier.bibliographicCitation | PARASITOLOGY RESEARCH, v.101, no.5, pp 1225 - 1231 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000249407400008 | - |
dc.identifier.scopusid | 2-s2.0-34548563717 | - |
dc.citation.endPage | 1231 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1225 | - |
dc.citation.title | PARASITOLOGY RESEARCH | - |
dc.citation.volume | 101 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | SCHISTOSOMA-MANSONI | - |
dc.subject.keywordPlus | FASCIOLA-HEPATICA | - |
dc.subject.keywordPlus | 3-DIMENSIONAL STRUCTURE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | VACCINATION | - |
dc.subject.keywordPlus | JAPONICUM | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordPlus | CDNA | - |
dc.relation.journalResearchArea | Parasitology | - |
dc.relation.journalWebOfScienceCategory | Parasitology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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