Abundance of aryl hydrocarbon receptor potentiates benzo[a]pyrene-induced apoptosis in Hepa1c1c7 cells via CYP1A1 activation
DC Field | Value | Language |
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dc.contributor.author | Chung, Jin-Yong | - |
dc.contributor.author | Kim, Ji Young | - |
dc.contributor.author | Kim, Won Rok | - |
dc.contributor.author | Lee, Seung Gee | - |
dc.contributor.author | Kim, Yoon-Jae | - |
dc.contributor.author | Park, Ji-Eun | - |
dc.contributor.author | Hong, Yeon-Pyo | - |
dc.contributor.author | Chun, Young-Jin | - |
dc.contributor.author | Park, Young Chul | - |
dc.contributor.author | Oh, Seunghoon | - |
dc.contributor.author | Yoo, Ki Soo | - |
dc.contributor.author | Yoo, Young Hyun | - |
dc.contributor.author | Kim, Jong-Min | - |
dc.date.available | 2019-05-30T06:32:18Z | - |
dc.date.issued | 2007-06 | - |
dc.identifier.issn | 0300-483X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24053 | - |
dc.description.abstract | Although B[a]P-induced apoptosis has been demonstrated in Hepa1c1c7 cells, the cellular signaling pathway(s) by which benzo[a]pyrene (B[a]P) elicits a cytotoxicity-mediated apoptogenic role remains to be elucidated. In this study, we showed that B[a]P induces apoptosis in a p53-mediated and caspase- 3 -dependent manner, which relates to the accumulation of the S phase of the cell cycle. Importantly, we have shown for the first time that Hepa1c1c7 cells retain a considerably high content of aryl hydrocarbon receptor (AhR) protein before B[a]P exposure, assuming that this status enables the cells to respond to B[a]P more readily as well as more efficiently. B[a]P treatment resulted in the downregulation of AhR and induced cytochrome P450 1A1 (CYP1A1) (but not cytochrome P450 1B1 (CYP1B1)) expression and activity. While (alpha-naphtoflavone (alpha-NF) and ellipticine suppressed B[a]P-induced CYP1A1 activation as well as apoptosis, the 2,3,4,5'-tetramethoxystilbene (TMS) and pyrene, known CYP1B1 inhibitors, failed to inhibit apoptosis. However, alpha-NF alone significantly increased CYP1A1 protein expression but not its activity, suggesting that alpha-NF more likely works as an AhR agonist in Hepa1c1c7 cells after B[a]P, rather than a direct inhibitor of CYP1A1 activity. In conclusion, it is suggested that the abundance of endogenous AhR level is an indispensable condition for an efficient cellular signaling of B[a]P and that control of AhR activity in Hepa1c1c7 cells might be important to cell fate resulting from CYP1A1 activation after B[a]P. (C) 2007 Elsevier Ireland Ltd. All rights reserved. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.title | Abundance of aryl hydrocarbon receptor potentiates benzo[a]pyrene-induced apoptosis in Hepa1c1c7 cells via CYP1A1 activation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.tox.2007.03.013 | - |
dc.identifier.bibliographicCitation | TOXICOLOGY, v.235, no.1-2, pp 62 - 72 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000247046600006 | - |
dc.identifier.scopusid | 2-s2.0-34247631090 | - |
dc.citation.endPage | 72 | - |
dc.citation.number | 1-2 | - |
dc.citation.startPage | 62 | - |
dc.citation.title | TOXICOLOGY | - |
dc.citation.volume | 235 | - |
dc.type.docType | Article | - |
dc.publisher.location | 아일랜드 | - |
dc.subject.keywordAuthor | AhR | - |
dc.subject.keywordAuthor | CYP1A1 | - |
dc.subject.keywordAuthor | CYP1B1 | - |
dc.subject.keywordAuthor | benzo[a]pyrene | - |
dc.subject.keywordAuthor | hepatoma cells | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordPlus | AH-RECEPTOR | - |
dc.subject.keywordPlus | ALPHA-NAPHTHOFLAVONE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | G(1) ARREST | - |
dc.subject.keywordPlus | DNA-REPAIR | - |
dc.subject.keywordPlus | P53 | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | CYTOCHROME-P450 | - |
dc.subject.keywordPlus | PROCARCINOGENS | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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