Differential gene expression by styrene in rat reproductive tissue
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Jee Hye | - |
dc.contributor.author | Choi, Chang-Su | - |
dc.contributor.author | Kim, Mie Young | - |
dc.contributor.author | Chun, Young-Jin | - |
dc.date.available | 2019-05-30T06:37:30Z | - |
dc.date.issued | 2007-01 | - |
dc.identifier.issn | 1528-7394 | - |
dc.identifier.issn | 1087-2620 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24207 | - |
dc.description.abstract | Styrene is an important industrial chemical that is extensively used in the production of resins, rubbers and fiberglass-reinforced plastics. Exposing male rats to high doses of styrene may produce sperm abnormalities or infertility. To determine the mechanism underlying styrene-mediated toxicity in male reproductive organs, a reverse transcription-polymerase chai reaction (RT-PCR) technology was employed using annealing control primers (ACPs) to identify the differentially expressed genes following styrene treatment in isolated testis of male rats. By using 120 ACPs, a total of 6 expressed sequence tags (ESTs) of genes were differentially expressed in styrene-treated rats, as compare to untreated, which were cloned and sequenced. Of the genes analyzed, 5 genes (testis-specific expressed gene 101, protein kinase C, H+-ATPase isoform 2, peroxiredoxin 1, and aquaporin 9) were inducible and one gene expression (clusterin) was significantly suppressed by styrene. Regulation of each gene by styrene was confirmed by RT-PCR. It was shown that styrene decreased clusterin expression in a concentration-dependent manner and these effects occurred mainly in testis. Taken together, these results indicate that repression of clusterin gene expression by styrene may play an important role in styrene-mediated toxicities. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | TAYLOR & FRANCIS INC | - |
dc.title | Differential gene expression by styrene in rat reproductive tissue | - |
dc.type | Article | - |
dc.identifier.doi | 10.1080/15287390701434414 | - |
dc.identifier.bibliographicCitation | JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v.70, no.15-16, pp 1259 - 1263 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000248414300003 | - |
dc.identifier.scopusid | 2-s2.0-34547193344 | - |
dc.citation.endPage | 1263 | - |
dc.citation.number | 15-16 | - |
dc.citation.startPage | 1259 | - |
dc.citation.title | JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES | - |
dc.citation.volume | 70 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | CONTROL PRIMER SYSTEM | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | EXPOSED WORKERS | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | CLUSTERIN | - |
dc.subject.keywordPlus | PEROXIREDOXINS | - |
dc.subject.keywordPlus | GLYCOPROTEIN | - |
dc.subject.keywordPlus | BIOMARKERS | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | SP-40,40 | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalResearchArea | Public, Environmental & Occupational Health | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.relation.journalWebOfScienceCategory | Public, Environmental & Occupational Health | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.