Activation of p38 MAPK is involved in endothelin-1-stimulated COX-2 expression in cultured feline esophageal smooth muscle cells
DC Field | Value | Language |
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dc.contributor.author | Song, Hyun Ju | - |
dc.contributor.author | Min, Young Sil | - |
dc.contributor.author | Shin, Chang Yell | - |
dc.contributor.author | Jeong, Ji Hoon | - |
dc.contributor.author | Sohn, Uy Dong | - |
dc.date.available | 2019-05-30T06:40:09Z | - |
dc.date.issued | 2006-08 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24293 | - |
dc.description.abstract | We investigated the possible role of p38 MAPK and ETB receptors in ET-1 induction of cyclooxygenase-2 (COX-2) and prostaglandin E-2 (PGE(2)) in cultured feline esophageal smooth muscle cells (ESMC). Confluent layers of ESMC were stimulated with 10 nM ET-1 and expression of COX-1 and COX-2, involvement of receptors, and activation of p38 MAPK, were examined by Western blot analysis. Levels of PGE2 induced by ET-1 were measured by Elisa. Using ETA and ETB antagonists (BQ-123 and BQ-788, respectively), the contribution of the ET receptors to COVI and COX-2 expression induced by ET-1 was determined. Western blot analysis revealed that treatment of ESMC with ET-1 resulted in transient expression of COX-2 and activation of p38 MAPK. Activation of p38 MAPK was maximal after 1 h. S13202190, a p38 MAPK inhibitor, reduced expression of COX-2, but not COX-1. ET-1-induced release of PGE2 was also blocked by S13202190. COX-2 expression was upregulated only via the ETB receptor, and COX-1 expression was not affected by either antagonist. Taken together, our data suggest that ET-1 causes p38 MAPK-dependent expression of COX-2 by interacting with ETB receptors on ESMC. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | Activation of p38 MAPK is involved in endothelin-1-stimulated COX-2 expression in cultured feline esophageal smooth muscle cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/aoc.1009 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.22, no.1, pp 44 - 50 | - |
dc.identifier.kciid | ART001022049 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000240323200007 | - |
dc.identifier.scopusid | 2-s2.0-33748319438 | - |
dc.citation.endPage | 50 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 44 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 22 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | COX | - |
dc.subject.keywordAuthor | esophageal smooth muscle cells | - |
dc.subject.keywordAuthor | ET-1 | - |
dc.subject.keywordAuthor | ETB receptor | - |
dc.subject.keywordAuthor | p38 MAPK | - |
dc.subject.keywordAuthor | PGE(2) | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | GLOMERULAR MESANGIAL CELLS | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | COMMON BILE-DUCT | - |
dc.subject.keywordPlus | FACTOR-KAPPA-B | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 EXPRESSION | - |
dc.subject.keywordPlus | ENDOTHELIN RECEPTOR | - |
dc.subject.keywordPlus | NUCLEAR-FACTOR | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | PHOSPHOLIPASE A(2) | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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