Hirsutenone inhibits phorbol ester-induced upregulation of COX-2 and MMP-9 in cultured human mammary epithelial cells: NF-kappa B as a potential molecular target
DC Field | Value | Language |
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dc.contributor.author | Kim, JH | - |
dc.contributor.author | Lee, KW | - |
dc.contributor.author | Lee, MW | - |
dc.contributor.author | Lee, HJ | - |
dc.contributor.author | Kim, SH | - |
dc.contributor.author | Surh, YJ | - |
dc.date.available | 2019-05-30T07:34:13Z | - |
dc.date.issued | 2006-01-23 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.issn | 1873-3468 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24428 | - |
dc.description.abstract | Inappropriate upregulation of cyclooxygenase-2 (COX-2) and matrix metalloproteinases (MMPs) has been implicated in the pathogenesis of various types of cancer. In the present study, we investigated the effects of hirsutenone, a diarylheptanoid isolated from the medicinal plant Alnus hirsuta var. sibirica, on the expression of COX-2 and MMP-9 induced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) in MCF10A human breast epithelial cells. Treatment of MCF10A cells with TPA led to the upregulation of COX-2 and MMP-9. Hirsutenone at 12 mu M inhibited the TPA-induced COX-2 expression at both the transcriptional and posttranscriptional levels. Hirsutenone also suppressed the synthesis of prostaglandin E-2, one of the major products of COX-2, and its catalytic activity. The upregulation of MMP-9 by TPA was also significantly reduced by hirsutenone. Likewise, hirsutenone attenuated the invasiveness and motility of MCF10A cells stimulated with TPA. Hirsutenone blocked the TPA-induced DNA binding of nuclear factor kappa B (NF-kappa B) and translocation of p65, the functionally active NF-kappa B subunit, to the nucleus. The luciferase reporter gene assay revealed that hirsutenone abrogated the transcriptional activity of NF-kappa B. Treatment of MCF10A cells with N-alpha-TOSYI-L-phenylaianine chloromethyl ketone, a specific inhibitor of NF-kappa B, reduced the TPA-induced expression of COX-2 and MMP-9. In summary, hirsutenone inhibits the TPA-induced upregulation of COX-2 and MMP-9 in human breast epithelial cells, possibly by targeting NF-kappa B, which may contribute to its chemopreventive effects. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Hirsutenone inhibits phorbol ester-induced upregulation of COX-2 and MMP-9 in cultured human mammary epithelial cells: NF-kappa B as a potential molecular target | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.febslet.2005.12.051 | - |
dc.identifier.bibliographicCitation | FEBS LETTERS, v.580, no.2, pp 385 - 392 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000234937400007 | - |
dc.identifier.scopusid | 2-s2.0-30644461913 | - |
dc.citation.endPage | 392 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 385 | - |
dc.citation.title | FEBS LETTERS | - |
dc.citation.volume | 580 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | hirsutenone | - |
dc.subject.keywordAuthor | cyclooxygenase-2 | - |
dc.subject.keywordAuthor | matrix metalloprotemase-9 | - |
dc.subject.keywordAuthor | human breast epithelial cells | - |
dc.subject.keywordAuthor | nuclear factor kappa B | - |
dc.subject.keywordAuthor | invasion | - |
dc.subject.keywordAuthor | migration | - |
dc.subject.keywordPlus | NONSTEROIDAL ANTIINFLAMMATORY DRUGS | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 EXPRESSION | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | HUMAN MONOCYTES | - |
dc.subject.keywordPlus | PKC ACTIVATION | - |
dc.subject.keywordPlus | IV COLLAGENASE | - |
dc.subject.keywordPlus | ALNUS-HIRSUTA | - |
dc.subject.keywordPlus | P38 MAPK | - |
dc.subject.keywordPlus | CHEMOPREVENTION | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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