Endogenous level of 28-norcastasterone is strictly regulated in plant cells
- Authors
- Kim, Tae-Wuk; Hwang, Jung-Yun; Joo, Se-Hwan; Cheong, Hyeonsook; Pharis, Richard P.; Kim, Seong-Ki
- Issue Date
- Dec-2005
- Publisher
- BOTANICAL SOCIETY OF KOREA
- Keywords
- Arabidopsis; brassinosteroid; homeostatic regulation; 28-norcastasterone; Phaseolus vulgaris
- Citation
- JOURNAL OF PLANT BIOLOGY, v.48, no.4, pp 483 - 486
- Pages
- 4
- Journal Title
- JOURNAL OF PLANT BIOLOGY
- Volume
- 48
- Number
- 4
- Start Page
- 483
- End Page
- 486
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24455
- DOI
- 10.1007/BF03030591
- ISSN
- 1226-9239
1867-0725
- Abstract
- A cell-free enzyme solution prepared from cultured cells of Phaseolus vulgaris mediated C-24 methylation of 28-norcastasterone to castasterone with the aid of S-adenosylmethionine as a co-substrate in the presence of the NADPH cofactor. This enzyme solution also catalyzed conversion of 28-norcastasterone to a demethylated 28-norcastasterone, most likely 26,28-didemethyl-castasterone, when S-adenosylmethionine was not added to the enzyme solution. Furthermore, gene expression of Arabidopsis CYP85A1 and CYP85A2 mediating the conversion of 6-deoxo-28-norcastasterone to 28-norcastasterone was strongly inhibited by treatment of 28-norcastasterone. These results suggest that 28-norcastasterone, along with castasterone and brassinolide, is an important brassinosteroid whose endogenous level should be strictly controlled to express brassinosteroid activities in plants.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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