Expression and modulation of LL-37 in normal human keratinocytes, HaCaT cells, and inflammatory skin diseases
DC Field | Value | Language |
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dc.contributor.author | Kim, JE | - |
dc.contributor.author | Kim, BJ | - |
dc.contributor.author | Jeong, MS | - |
dc.contributor.author | Seo, SJ | - |
dc.contributor.author | Kim, MN | - |
dc.contributor.author | Hong, CK | - |
dc.contributor.author | Ro, BI | - |
dc.date.available | 2019-05-30T07:37:19Z | - |
dc.date.issued | 2005-08 | - |
dc.identifier.issn | 1011-8934 | - |
dc.identifier.issn | 1598-6357 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24553 | - |
dc.description.abstract | Defensins and cathelicidins (LL-37) are major antimicrobial peptides (AMPs) of the innate immune system of the human skin. In normal non-inflamed skin these peptides are negligible, but their expression can be markedly increased in inflammatory skin disease such as psoriasis. We designed this study to identify the expressions of LL-37 in normal human keratinocyte (NHK) and HaCaT cells after exposure to stimulants and to investigate difference of LL-37 expression accompanied with cell differentiation status, and come to understand difference of susceptibility to infection in atopic dermatitis and psoriasis. Expressions of LL-37 in NHKs and HaCaT cells were evaluated by using RT-PCR, Western blotting, and immunohistochemical (IHC) staining at 6, 12, and 24 hr post stimulation after exposure to Ultraviolet B irradiation and lipopolysaccharide. And expression of LL-37 in skin biopsy specimens from patients with atopic dermatitis and psoriasis was determined by immunohistochemical analysis. In time-sequential analyses of LL-37 expression revealed that LL-37 was expressed in NHKs, but not in HaCaT cells. IHC analysis confirmed the presence of abundant LL-37 in the epidermis of psoriasis. Therefore we deduced that expression of LL-37 is affected by UV irradiation, bacterial infection, and status of cell differentiation. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN ACAD MEDICAL SCIENCES | - |
dc.title | Expression and modulation of LL-37 in normal human keratinocytes, HaCaT cells, and inflammatory skin diseases | - |
dc.type | Article | - |
dc.identifier.doi | 10.3346/jkms.2005.20.4.649 | - |
dc.identifier.bibliographicCitation | JOURNAL OF KOREAN MEDICAL SCIENCE, v.20, no.4, pp 649 - 654 | - |
dc.identifier.kciid | ART000967559 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000231633500021 | - |
dc.identifier.scopusid | 2-s2.0-24644496354 | - |
dc.citation.endPage | 654 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 649 | - |
dc.citation.title | JOURNAL OF KOREAN MEDICAL SCIENCE | - |
dc.citation.volume | 20 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | CAP18 lipopolysaccharide-binding protein | - |
dc.subject.keywordAuthor | LL-37 | - |
dc.subject.keywordAuthor | keratinocytes | - |
dc.subject.keywordAuthor | HaCaT cell | - |
dc.subject.keywordAuthor | psoriasis | - |
dc.subject.keywordAuthor | dermatitis | - |
dc.subject.keywordAuthor | atopic | - |
dc.subject.keywordPlus | CATHELICIDIN ANTIMICROBIAL PEPTIDES | - |
dc.subject.keywordPlus | INVASIVE BACTERIAL-INFECTION | - |
dc.subject.keywordPlus | ATOPIC-DERMATITIS | - |
dc.subject.keywordPlus | SALIVARY-GLANDS | - |
dc.subject.keywordPlus | BETA-DEFENSINS | - |
dc.subject.keywordPlus | INNATE DEFENSE | - |
dc.subject.keywordPlus | IMMUNITY | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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