Characterization of the functional domains of human foamy virus integrase using chimeric integrases
- Authors
- Lee, Hak Sung; Kang, Seung Yi; Shin, Cha-Gyun
- Issue Date
- Apr-2005
- Publisher
- SPRINGER SINGAPORE PTE LTD
- Keywords
- chimeric integrase; defective point mutants; functional domain
- Citation
- MOLECULES AND CELLS, v.19, no.2, pp 246 - 255
- Pages
- 10
- Journal Title
- MOLECULES AND CELLS
- Volume
- 19
- Number
- 2
- Start Page
- 246
- End Page
- 255
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24629
- ISSN
- 1016-8478
0219-1032
- Abstract
- Retroviral integrases insert viral DNA into target DNA. In this process they recognize their own DNA specifically via functional domains. In order to analyze these functional domains, we constructed six chimeric integrases by swapping domains between HIV-1 and HFV integrases, and two point mutants of HFV integrase. Chimeric integrases with the central domain of HIV-1 integrase had strand transfer and disintegration activities, in agreement with the idea that the central domain determines viral DNA specificity and has catalytic activity. On the other hand, chimeric integrases with the central domain of HFV integrase did not have any enzymatic activity apart from FFH that had weak disintegration activity, suggesting that the central domain of HFV integrase was defective catalytically or structurally. However, these inactive chimeras were efficiently complemented by the point mutants (D164A and E200A) of HFV integrase, indicating that the central domain of HFV integrase possesses potential enzymatic activity but is not able to recognize viral or target DNA without the help of its homologous N-terminal and C-terminal domains.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24629)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.