Overexpression of squalene synthase in Eleutherococcus senticosus increases phytosterol and triterpene accumulation
DC Field | Value | Language |
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dc.contributor.author | Seo, Jin-Wook | - |
dc.contributor.author | Jeong, Jae-Hun | - |
dc.contributor.author | Shin, Cha-Gyun | - |
dc.contributor.author | Lo, Seog-Cho | - |
dc.contributor.author | Han, Seong-Soo | - |
dc.contributor.author | Yu, Ki-Won | - |
dc.contributor.author | Harada, Emiko | - |
dc.contributor.author | Han, Jeong-Yeon | - |
dc.contributor.author | Choi, Yong-Eui | - |
dc.date.available | 2019-05-30T07:39:25Z | - |
dc.date.issued | 2005-04 | - |
dc.identifier.issn | 0031-9422 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24632 | - |
dc.description.abstract | Squalene synthase (SS) catalyzes the first committed step in sterol and triterpenoid biosynthesis. Transgenic Eleutherococcus senticosus Rupr. and Maxim. plants were generated by introducing an SS-encoding gene derived from Panax ginseng (PgSS1) together with genes expressing hygromycin phosphotransferase and green fluorescent protein (GFP) through Agrobacterium-mediated transformation. Early globular embryo clusters developing from the embryogenic callus were used for Agrobacteritan-mediated transformation. Transformants were selected on Murashige Skoog medium containing 25 mg/L hygromycin. Hygromycin-resistant somatic embryos developed into plants after the cotyledonary embryos were treated with 14.4 μ M gibberellic acid. Transformation was confirmed by polymerase chain reaction, Southern, and GFP analyses. The SS enzyme activity of the transgenic plants was up to 3-fold higher than that of wild-type plants. In addition, GC-MS and HPLC analysis revealed that phytosterols (P-sitosterol and stigmasterol) as well as triterpene saponins (ciwujianosides B (1), C-1 (2), C-2 (3), C-3 (4), C-4 (5), D-1 (6) and D-2 (7)) levels in transgenic E senticostis were increased by 2- to 2.5-fold. These results suggest that the metabolic engineering of E. senticosus to enhance production of phytosterols and triterpenoids by introducing the PgSS1 gene was successfully achieved by Agrobacterium-mediated genetic transformation. © 2005 Elsevier Ltd. All rights reserved. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Overexpression of squalene synthase in Eleutherococcus senticosus increases phytosterol and triterpene accumulation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.phytochem.2005.02.016 | - |
dc.identifier.bibliographicCitation | PHYTOCHEMISTRY, v.66, no.8, pp 869 - 877 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000228942000003 | - |
dc.identifier.scopusid | 2-s2.0-17444400413 | - |
dc.citation.endPage | 877 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 869 | - |
dc.citation.title | PHYTOCHEMISTRY | - |
dc.citation.volume | 66 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Eleutherococcus senticosus | - |
dc.subject.keywordAuthor | Siberian ginseng | - |
dc.subject.keywordAuthor | Araliaceae | - |
dc.subject.keywordAuthor | Ciwujianoside | - |
dc.subject.keywordAuthor | genetic transformation | - |
dc.subject.keywordAuthor | squalene synthase | - |
dc.subject.keywordAuthor | triterpene biosynthesis | - |
dc.subject.keywordPlus | FUNGAL ELICITOR | - |
dc.subject.keywordPlus | CALLUS TISSUES | - |
dc.subject.keywordPlus | TOBACCO | - |
dc.subject.keywordPlus | SAPONINS | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | STEROLS | - |
dc.subject.keywordPlus | SESSILIFLORUS | - |
dc.subject.keywordPlus | SUPPRESSION | - |
dc.subject.keywordPlus | CULTURES | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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