Catabolic role of a three-component salicylate oxygenase from Sphingomonas yanoikuyae B1 in polycyclic aromatic hydrocarbon degradation
DC Field | Value | Language |
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dc.contributor.author | Cho, Okyoung | - |
dc.contributor.author | Choi, Ki Young | - |
dc.contributor.author | Zylstra, Gerben J. | - |
dc.contributor.author | Kim, Young-Soo | - |
dc.contributor.author | Kim, Seong-Ki | - |
dc.contributor.author | Lee, Joon H. | - |
dc.contributor.author | Sohn, Ho-Yong | - |
dc.contributor.author | Kwon, Gi-Seok | - |
dc.contributor.author | Kim, Young Min | - |
dc.contributor.author | Kim, Eungbin | - |
dc.date.available | 2019-05-30T08:32:30Z | - |
dc.date.issued | 2005-02 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24661 | - |
dc.description.abstract | Sphingomonas yanoikuyae B1 possesses several different multicomponent oxygenases involved in metabolizing aromatic compounds. Six different pairs of genes encoding large and small subunits of oxygenase iron-sulfur protein components have previously been identified in a gene cluster involved in the degradation of both monocyclic and polycyclic aromatic hydrocarbons. Insertional inactivation of one of the oxygenase large subunit genes, bphA1c, results in a mutant strain unable to grow on naphthalene, phenanthrene, or salicylate. The knockout mutant accumulates salicylate from naphthalene and 1-hydroxy-2-naphthoic acid from phenanthrene indicating the loss of salicylate oxygenase activity. Complementation experiments verify that the salicylate oxygenase in S. yanoikuyae B1 is a three-component enzyme consisting of an oxygenase encoded by bph A2cA1c, a ferredoxin encoded by the adjacent bphA3, and a ferredoxin reductase encoded by bphA4 located over 25 kb away. Expression of bphA3-bphA2c-bphA1c genes in Escherichia coli demonstrated the ability of salicylate oxygenase to convert salicylate to catechol and 3-, 4-, and 5-methylsalicylate to methylcatechols. (C) 2004 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Catabolic role of a three-component salicylate oxygenase from Sphingomonas yanoikuyae B1 in polycyclic aromatic hydrocarbon degradation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbrc.2004.12.060 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.327, no.3, pp 656 - 662 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000226674800005 | - |
dc.identifier.scopusid | 2-s2.0-19944428529 | - |
dc.citation.endPage | 662 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 656 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 327 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Sphingomonas | - |
dc.subject.keywordAuthor | salicylate oxygenase | - |
dc.subject.keywordAuthor | phenanthrene | - |
dc.subject.keywordAuthor | naphthalene | - |
dc.subject.keywordAuthor | biphenyl | - |
dc.subject.keywordAuthor | xylene | - |
dc.subject.keywordPlus | M-XYLENE DEGRADATION | - |
dc.subject.keywordPlus | SP STRAIN B1 | - |
dc.subject.keywordPlus | FUNCTIONAL-ANALYSIS | - |
dc.subject.keywordPlus | BIPHENYL | - |
dc.subject.keywordPlus | HYDROXYLASE | - |
dc.subject.keywordPlus | OXIDATION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | DIOXYGENASES | - |
dc.subject.keywordPlus | NAPHTHALENE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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