Confirmation of Vpr as a fibrinolytic enzyme present in extracellular proteins of Bacillus subtilis
DC Field | Value | Language |
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dc.contributor.author | Kho, CW | - |
dc.contributor.author | Park, SG | - |
dc.contributor.author | Cho, S | - |
dc.contributor.author | Lee, DH | - |
dc.contributor.author | Myung, PK | - |
dc.contributor.author | Park, BC | - |
dc.date.available | 2019-05-30T08:33:22Z | - |
dc.date.issued | 2005-01 | - |
dc.identifier.issn | 1046-5928 | - |
dc.identifier.issn | 1096-0279 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24689 | - |
dc.description.abstract | We have previously reported a proteomic approach to detect fibrinolytic enzymes from the secreted proteins of Bacillis subtilis 168 and identified two extracellular fibrinolytic enzymes of Bacillus. namely, Vpr and WprA. In this study. to confirm the fibrinolytic activity of Vpr, we cloned the vpr gene and expressed it in Escherichia coli, where it is predominantly localized to inclusion bodies. After affinity purification and desalting steps, the expressed Vpr is auto-processed to an active form. Interestingly. after the desalting step, several additional bands with fibrinolytic activity were detected in zymography gel along with a mature form (68 kDa) of Vpr. MALDI-TOF analyses of these bands revealed that Vpr could exist in multiple forms. (C) 2004 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Confirmation of Vpr as a fibrinolytic enzyme present in extracellular proteins of Bacillus subtilis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.pep.2004.08.008 | - |
dc.identifier.bibliographicCitation | PROTEIN EXPRESSION AND PURIFICATION, v.39, no.1, pp 1 - 7 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000226153600001 | - |
dc.identifier.scopusid | 2-s2.0-10644227920 | - |
dc.citation.endPage | 7 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.citation.volume | 39 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | serine protease | - |
dc.subject.keywordAuthor | Vpr | - |
dc.subject.keywordAuthor | zymography | - |
dc.subject.keywordAuthor | mass spectrometry | - |
dc.subject.keywordAuthor | fibrinolytic enzymes | - |
dc.subject.keywordPlus | SERINE PROTEASE | - |
dc.subject.keywordPlus | ZYMOGRAPHY | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | GELS | - |
dc.subject.keywordPlus | BACILLUS-SUBTILIS-168 | - |
dc.subject.keywordPlus | METALLOPROTEASE | - |
dc.subject.keywordPlus | FORMS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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