Inhibitory effects of epicatechin on interleukin-1 beta-induced inducible nitric oxide synthase expression in RINm5F cells and rat pancreatic islets by down-regulation of NF-kappa B activation
DC Field | Value | Language |
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dc.contributor.author | Kim, MJ | - |
dc.contributor.author | Ryu, GR | - |
dc.contributor.author | Kang, JH | - |
dc.contributor.author | Sim, SS | - |
dc.contributor.author | Min, DS | - |
dc.contributor.author | Rhie, DJ | - |
dc.contributor.author | Yoon, SH | - |
dc.contributor.author | Hahn, SJ | - |
dc.contributor.author | Jeong, IK | - |
dc.contributor.author | Hong, KJ | - |
dc.contributor.author | Kim, MS | - |
dc.contributor.author | Jo, YH | - |
dc.date.available | 2019-05-30T08:34:47Z | - |
dc.date.issued | 2004-11 | - |
dc.identifier.issn | 0006-2952 | - |
dc.identifier.issn | 1873-2968 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24741 | - |
dc.description.abstract | Cytokines that are released by infiltrating inflammatory cells around the pancreatic islets are involved in the pathogenesis of type 1 diabetes mellitus. Specifically, interleukin-1beta (IL-1beta) stimulates inducible nitric oxide synthase (iNOS) expression and nitric oxide overproduction, leading to the beta-cell damage. In activating this pathway, nuclear factor-kappaB (NF-kappaB) plays a crucial role, and many of the IL-1beta-sensitive genes contain NF-kappaB binding sites in their promoter regions. We have recently shown that epicatechin, which is a flavonoid, had a protective effect on pancreatic beta-cells in both streptozotocin-treated rats and islets. In the present study, the effects of epicatechin on IL-1beta-induced beta-cell damage were examined. RINm5F cells and islets were pretreated with epicatechin and next incubated with IL-1beta. The released nitrite, iNOS protein and mRNA expression levels were then measured. IkappaBalpha protein, nuclear translocation of NF-kappaB, and NF-kappaB DNA binding activity were also determined. Following the transient transfection of an iNOS promoter into the cells, the iNOS promoter activity was measured. ATP- or D-glucose-induced insulin release was measured in RINm5F cells and islets, respectively. Epicatechin significantly reduced IL-1beta-induced nitrite production, iNOS protein and mRNA expressions, and it also inhibited IL-1beta-induced IkappaBalpha protein degradation, NF-kappaB activation, and iNOS promoter activity. Epicatechin partly restored the IL-1beta-induced inhibition of insulin release. These results suggest that epicatechin inhibits the IL-1beta-induced iNOS expression by down-regulating NF-kappaB activation, and protecting beta-cells from IL-1beta. (C) 2004 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Inhibitory effects of epicatechin on interleukin-1 beta-induced inducible nitric oxide synthase expression in RINm5F cells and rat pancreatic islets by down-regulation of NF-kappa B activation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bcp.2004.06.031 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL PHARMACOLOGY, v.68, no.9, pp 1775 - 1785 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000224443000010 | - |
dc.identifier.scopusid | 2-s2.0-4644237615 | - |
dc.citation.endPage | 1785 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1775 | - |
dc.citation.title | BIOCHEMICAL PHARMACOLOGY | - |
dc.citation.volume | 68 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | epicathechin | - |
dc.subject.keywordAuthor | interleukin-1 beta | - |
dc.subject.keywordAuthor | iNOS | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | RINm5F | - |
dc.subject.keywordAuthor | islet | - |
dc.subject.keywordPlus | INSULIN-PRODUCING CELLS | - |
dc.subject.keywordPlus | ENZYME GENE-EXPRESSION | - |
dc.subject.keywordPlus | BETA-CELLS | - |
dc.subject.keywordPlus | PROTEIN-SYNTHESIS | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | (-)-EPICATECHIN | - |
dc.subject.keywordPlus | STREPTOZOTOCIN | - |
dc.subject.keywordPlus | INVOLVEMENT | - |
dc.subject.keywordPlus | DESTRUCTION | - |
dc.subject.keywordPlus | LANGERHANS | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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