Involvement of Akt in mitochondria-dependent apoptosis induced by a cdc25 phosphatase inhibitor naphthoquinone analog
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, HJ | - |
dc.contributor.author | Kang, SK | - |
dc.contributor.author | Mun, JY | - |
dc.contributor.author | Chun, YJ | - |
dc.contributor.author | Choi, KH | - |
dc.contributor.author | Kim, MY | - |
dc.date.available | 2019-05-30T08:39:25Z | - |
dc.date.issued | 2003-12 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.issn | 1873-3468 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24912 | - |
dc.description.abstract | Vitamin K-related analogs induce growth inhibition via a cell cycle arrest through cdc25A phosphatase inhibition in various cancer cell tines. We report that 2,3-dichloro-5,8-dihydroxy-1,4-naphthoquinone (DDN), a naphthoquinone analog, induces mitochondria-dependent apoptosis in human promyelocytic leukemia HL-60 cells. DDN induced cytochrome c release, Bax translocation, cleavage of Bid and Bad, and activation of caspase-3, -8, -9 upon the induction of apoptosis. Cleavage of Bid, the caspase-8 substrate, was inhibited by the broad caspase inhibitor z-Val-Ala-Asp(OMe)-fluoromethylketone (zVAD-fmk), whereas cytochrome c release was not affected, suggesting that activation of caspase-8 and subsequent Bid cleavage occur downstream of cytochrome c release. DDN inhibited the activation of Akt detected by decreasing level of phosphorylation. Overexpression of constitutively active Akt protected cells from DDN-induced apoptosis, while dominant negative Akt moderately enhanced cell death. Furthermore, Akt prevented release of cytochrome c and cleavage of Bad in DDN-treated HL-60 cells. Taken together, DDN-induced apoptosis is associated with mitochondrial signaling which involves cytochrome c release via a mechanism inhibited by Akt. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Involvement of Akt in mitochondria-dependent apoptosis induced by a cdc25 phosphatase inhibitor naphthoquinone analog | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/S0014-5793(03)01238-9 | - |
dc.identifier.bibliographicCitation | FEBS LETTERS, v.555, no.2, pp 217 - 222 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000187121400008 | - |
dc.identifier.scopusid | 2-s2.0-0344440758 | - |
dc.citation.endPage | 222 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 217 | - |
dc.citation.title | FEBS LETTERS | - |
dc.citation.volume | 555 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | naphthoquinone analog | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | cytochrome c | - |
dc.subject.keywordAuthor | Bad | - |
dc.subject.keywordAuthor | Akt | - |
dc.subject.keywordPlus | CYTOCHROME-C RELEASE | - |
dc.subject.keywordPlus | VITAMIN-K | - |
dc.subject.keywordPlus | BAX | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | INSERTION | - |
dc.subject.keywordPlus | CERAMIDE | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.