Phenolic compounds in the flesh of Korean apple cultivar, Busa

Abstract

Phenolic compounds were purified from Busa, the most widely cultivated apple cultivar in Korea, by a sequential separation employing PVPP, Amberlite XAD-2 and Sephadex LH-20 column chromatography. Phenolic compounds in some fractions were further identified by directly comparing with corresponding phenolic standards after separation on HPLC and GC-MS. The phenolic compounds identified were p-hydoxybenzoic, protocatechuic, 4-hydroxymetylbenzonic, caffeic, p-coumaric, o-coumatic,ferulic, sinapic and chlorogenic acids and (+/-)-catechin. Among them, 4-hydroxymethylbenzoic, protocatechuic, o-coumaric and sinapic acids were ascertained as new members of a family of phenolic acids present in apples. Also the presence of rutin, quercetin and phlorizin in apples were demonstrated using HPLC.