C-2-ceramide-induced circular smooth muscle cell contraction involves PKC-epsilon and p44/p42 MAPK activation in cat oesophagus
DC Field | Value | Language |
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dc.contributor.author | Shin, CY | - |
dc.contributor.author | Lee, YP | - |
dc.contributor.author | Lee, TS | - |
dc.contributor.author | Song, HJ | - |
dc.contributor.author | Sohn, UD | - |
dc.date.available | 2019-05-30T09:35:35Z | - |
dc.date.issued | 2002-11 | - |
dc.identifier.issn | 0898-6568 | - |
dc.identifier.issn | 1873-3913 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25070 | - |
dc.description.abstract | We investigated the mechanism of C-2-ceramide (C-2)-induced circular smooth muscle cell contraction in cat oesophagus. C-2 produced contraction of smooth muscle cells isolated by enzymatic digestion, peaked at 30 s and was sustained at a plateau at 5 min. The response to C-2 was concentration-dependent. H-7 or chelerythrine inhibited C-2-induced contraction, while the diacylglycerol (DAG) kinase inhibitor, R59949, had no effect, suggesting that the contraction is protein kinase C (PKC) pathway-dependent. To test if PKC-mediated contraction may be isozyme-specific, we examined the effects of PKC isozymes antibodies on contraction. PKC-epsilon antibody inhibited the contraction by C-2 but not by PKC-betaII or -gamma, suggesting that PKC-epsilon mediates the contraction by C-2. To characterize the specific PKC isozymes that mediate contraction of the smooth muscle cells, we used, as an inhibitor, N-myristoylated peptides (myr-PKC) derived from the pseudosubstrate sequences of PKC-alphabetagamma, -alpha, -delta, or -epsilon. myr-PKC-epsilon only inhibited the contraction, which was concentration-dependent, suggesting that PKC-epsilon isozyme is involved in the contraction. To examine which mitogen-activated protein kinases (MAPKs) are involved in C-2-induced contraction, specific MAPK inhibitors (MEK inhibitor, PD98059, and p38 MAPK inhibitor, SB202190) are used. Preincubation of PD98059 blocked the contraction induced by C-2 in a concentration-dependent manner. However, SB202190 had no effects on contraction. C-2 increased the intensity of the bands identified by phosphospecific p44/p42 MAPK antibody and preincubation of PD98059 decreased the intensity of bands as compared with C-2-stimulated cells. In conclusion, C-2 produced the contraction of smooth muscle cells of cat oesophagus. The contraction is mediated by PKC-epsilon, resulting in the activation of p44/p42 MAPK. (C) 2002 Elsevier Science Inc. All rights reserved. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.title | C-2-ceramide-induced circular smooth muscle cell contraction involves PKC-epsilon and p44/p42 MAPK activation in cat oesophagus | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/S0898-6568(02)00038-4 | - |
dc.identifier.bibliographicCitation | CELLULAR SIGNALLING, v.14, no.11, pp 925 - 932 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000178523000004 | - |
dc.identifier.scopusid | 2-s2.0-0036830407 | - |
dc.citation.endPage | 932 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 925 | - |
dc.citation.title | CELLULAR SIGNALLING | - |
dc.citation.volume | 14 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | C-2-ceramide | - |
dc.subject.keywordAuthor | PKC | - |
dc.subject.keywordAuthor | pseudosubstrate peptide | - |
dc.subject.keywordAuthor | MAP kinase | - |
dc.subject.keywordAuthor | cell contraction | - |
dc.subject.keywordPlus | PROTEIN-KINASE-C | - |
dc.subject.keywordPlus | LOWER ESOPHAGEAL SPHINCTER | - |
dc.subject.keywordPlus | SIGNAL-REGULATED KINASE | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | VASCULAR CELLS | - |
dc.subject.keywordPlus | CERAMIDE | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | TRANSDUCTION | - |
dc.subject.keywordPlus | SPHINGOLIPIDS | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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