The signal transduction of endothelin-1-induced circular smooth muscle cell contraction in cat esophagus
DC Field | Value | Language |
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dc.contributor.author | Shin, Chang Yell | - |
dc.contributor.author | Lee, Yul Pyo | - |
dc.contributor.author | Lee, Tai Sang | - |
dc.contributor.author | Je, Hyun Dong | - |
dc.contributor.author | Kim, Dong Seok | - |
dc.contributor.author | Sohn, Uy Dong | - |
dc.date.available | 2019-05-30T09:36:02Z | - |
dc.date.issued | 2002-09 | - |
dc.identifier.issn | 0022-3565 | - |
dc.identifier.issn | 1521-0103 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25085 | - |
dc.description.abstract | It has been known that endothelin-1 (ET-1) exerts important actions in gastrointestinal smooth muscle motility, but its precise mechanism remains unsolved. We investigated the intracellular mechanism of ET-1-induced circular smooth muscle cell contraction in cat esophagus. ET-1 produced contraction of smooth muscle cells isolated by enzymatic digestion. The contraction in response to ET-1 was concentration-dependent. Pertussis toxin (PTX) blocked contraction induced by ET-1 in intact cells. To identify the specific G protein involved in the contraction, muscle cells were permeabilized with saponin. The G(i3) or G(beta) protein antibody inhibited the contraction. Neomycin phospholipase C (PLC) inhibitor inhibited the contraction, but 7,7-dimethyleicosadienoic acid (phospholipase A(2) inhibitor) and p-chloromercuribenzoic acid (phospholipase D inhibitor) had no effects. Incubation of permeabilized cells with PLC-beta(3) isozyme antibody inhibited the contraction. 1-(5-Isoquinolinesulfonyl)- 2-methylpiperazine, chelerythrine [protein kinase C (PKC) inhibitor], or genistein (protein tyrosine kinase inhibitor) inhibited the contraction, but not by diacylglycerol (DAG) kinase inhibitor, R59949. To test whether the contraction may be PKC isozyme-specific, we examined the effect of PKC isozymes antibodies on the contraction. PKC-epsilon antibody inhibited the contraction. To characterize further the specific PKC isozymes that mediate the contraction, we used, as an inhibitor, N-myristoylated peptides (myr-PKC) derived from the pseudosubstrate sequences of PKC-alphabetagamma,- alpha,- delta, or -epsilon. myr-PKC-epsilon inhibited the contraction, confirming that PKC-epsilon isozyme is involved in the contraction. To examine whether mitogen-activated protein kinases (MAPKs) mediate the contraction, specific MAPK inhibitors [MAPK kinase inhibitor, PD98059, (2'-amino-3'-methoxyflavone), and p38 MAPK inhibitor, SB202190 (4-4-fluorophenyl) 2-(4-hydroxyphenyl)-5-(4-pyridyl) 1H-imidazole)] were used. PD98059 or SB202190 blocked the contraction. ET-1 increased the intensity of the detection bands identified by immunological methods as MAPK monoclonal p44/p42 peptides. PD98059 decreased the intensity of the detection bands compared with ET-1. In conclusion, ET-1-induced contraction in cat esophageal circular muscle cells depends on PTX-sensitive G(i3) protein and PLC-beta(3) isozyme, resulting in the activation of PKC-epsilon- or protein-tyrosine kinase-dependent pathway, subsequently mediating the activation of p44/p42 MAPK or p38 MAPK pathway. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS | - |
dc.title | The signal transduction of endothelin-1-induced circular smooth muscle cell contraction in cat esophagus | - |
dc.type | Article | - |
dc.identifier.doi | 10.1124/jpet.302.3.924 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, v.302, no.3, pp 924 - 934 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000177467100011 | - |
dc.identifier.scopusid | 2-s2.0-0036721270 | - |
dc.citation.endPage | 934 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 924 | - |
dc.citation.title | JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS | - |
dc.citation.volume | 302 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | PROTEIN-KINASE-C | - |
dc.subject.keywordPlus | RAT VENTRICULAR MYOCYTES | - |
dc.subject.keywordPlus | TYROSINE PHOSPHORYLATION | - |
dc.subject.keywordPlus | PHOSPHOLIPASE-C | - |
dc.subject.keywordPlus | ENDOTHELINLIKE IMMUNOREACTIVITY | - |
dc.subject.keywordPlus | DIFFERENTIAL ACTIVATION | - |
dc.subject.keywordPlus | SUBSEQUENT STIMULATION | - |
dc.subject.keywordPlus | MEDIATED ACTIVATION | - |
dc.subject.keywordPlus | CA-2+ RELEASE | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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