Use of internal standard RNA molecules for the RT-PCR amplification of the faeces-borne RNA viruses
DC Field | Value | Language |
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dc.contributor.author | Kim, K | - |
dc.contributor.author | Park, J | - |
dc.contributor.author | Chung, Y | - |
dc.contributor.author | Cheon, D | - |
dc.contributor.author | Lee, IB | - |
dc.contributor.author | Lee, S | - |
dc.contributor.author | Yoon, J | - |
dc.contributor.author | Cho, H | - |
dc.contributor.author | Song, C | - |
dc.contributor.author | Lee, KH | - |
dc.date.available | 2019-05-30T09:36:30Z | - |
dc.date.issued | 2002-07 | - |
dc.identifier.issn | 0166-0934 | - |
dc.identifier.issn | 1879-0984 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25104 | - |
dc.description.abstract | The diagnostic system based on reverse transcription (RT)-PCR has been used widely for the detection of viral genomes of faecal-borne RNA viruses. However, faecal specimens often produce both false positive and false negative results. Therefore, there is a need for a diagnosis procedure that can control for 'false-results'. In this study, an internal standard RNA that can serve as a non-competitive positive template was developed and used directly to detect faecal-borne RNA viruses without noticeable competitive inhibition of the target viral genome. These results suggest that the internal standard RNA is a useful standard molecule when undertaking diagnostic qualitative RT-PCR procedures for enteroviruses and related faecal-borne RNA viruses. (C) 2002 Elsevier Science B.V. All rights reserved. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Use of internal standard RNA molecules for the RT-PCR amplification of the faeces-borne RNA viruses | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/S0166-0934(02)00016-2 | - |
dc.identifier.bibliographicCitation | JOURNAL OF VIROLOGICAL METHODS, v.104, no.2, pp 107 - 115 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000177144500001 | - |
dc.identifier.scopusid | 2-s2.0-0036089868 | - |
dc.citation.endPage | 115 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 107 | - |
dc.citation.title | JOURNAL OF VIROLOGICAL METHODS | - |
dc.citation.volume | 104 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | internal standard RNA | - |
dc.subject.keywordAuthor | RT-PCR | - |
dc.subject.keywordAuthor | faeces-borne viruses | - |
dc.subject.keywordAuthor | diagnosis | - |
dc.subject.keywordPlus | CENTRAL-NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | POLYMERASE-CHAIN-REACTION | - |
dc.subject.keywordPlus | CEREBROSPINAL-FLUID | - |
dc.subject.keywordPlus | ASEPTIC-MENINGITIS | - |
dc.subject.keywordPlus | INFECTIONS | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ENTEROVIRUSES | - |
dc.subject.keywordPlus | MANAGEMENT | - |
dc.subject.keywordPlus | POLIOVIRUS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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