Protective effect of boldine on dopamine-induced membrane permeability transition in brain mitochondria and viability loss in PC12 cells
DC Field | Value | Language |
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dc.contributor.author | Youn, Young Chul | - |
dc.contributor.author | Kwon, Oh Sang | - |
dc.contributor.author | Han, Eun Sook | - |
dc.contributor.author | Song, Jin Ho | - |
dc.contributor.author | Shin, Yong Kyoo | - |
dc.contributor.author | Lee, Chung Soo | - |
dc.date.available | 2019-05-30T09:37:31Z | - |
dc.date.issued | 2002-02 | - |
dc.identifier.issn | 0006-2952 | - |
dc.identifier.issn | 1873-2968 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25143 | - |
dc.description.abstract | Boldine ([S]-2,9-dihydroxy-1,10-dimethoxyaporphine) has been shown to exert antioxidant and anti-inflammatory effects. The present study elucidated the protective effect of boldine on catecholamine-induced membrane permeability transition in brain mitochondria and viability loss in PC12 cells. Dopamine (200 muM) and 6-hydroxydopamine (6-OHDA, 100 muM) attenuated Ca2+ and succinate-induced mitochondrial swelling and membrane potential formation. Boldine (10-100 muM) and 10 mug/mL of superoxide dismutase (SOD) or catalase reduced the effect of catecholamine oxidation on brain mitochondria. Boldine, SOD, and catalase decreased catecholamine-induced mitochondrial cytochrome c release. Antioxidant enzymes attenuated the depressant effect of catecholamines on mitochondrial electron flow, whereas boldine did not reduce it. Boldine inhibited the catecholamine-induced decrease in thioredoxin reductase activity and the increase in thiol oxidation in mitochondria. It also showed a scavenging action on hydrogen peroxide and hydroxyl radicals and decreased the formation of melanin from dopamine. Boldine and antioxidant enzymes decreased the dopamine-induced cell death, including apoptosis, in PC12 cells. The results suggest that boldine may attenuate the catecholamine oxidation-induced brain mitochondrial dysfunction and decrease the dopamine-induced death of PC12 cells through a scavenging action on reactive oxygen species and inhibition of melanin formation and thiol oxidation. (C) 2002 Elsevier Science Inc. All rights reserved. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Protective effect of boldine on dopamine-induced membrane permeability transition in brain mitochondria and viability loss in PC12 cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/S0006-2952(01)00852-8 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL PHARMACOLOGY, v.63, no.3, pp 495 - 505 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000174506900016 | - |
dc.identifier.scopusid | 2-s2.0-0036471027 | - |
dc.citation.endPage | 505 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 495 | - |
dc.citation.title | BIOCHEMICAL PHARMACOLOGY | - |
dc.citation.volume | 63 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | boldine | - |
dc.subject.keywordAuthor | catecholamines | - |
dc.subject.keywordAuthor | brain mitochondria | - |
dc.subject.keywordAuthor | PC12 cells | - |
dc.subject.keywordAuthor | protection | - |
dc.subject.keywordPlus | CYTOCHROME-C RELEASE | - |
dc.subject.keywordPlus | MICROSOMAL LIPID-PEROXIDATION | - |
dc.subject.keywordPlus | RAT-LIVER MITOCHONDRIA | - |
dc.subject.keywordPlus | PARKINSONS-DISEASE | - |
dc.subject.keywordPlus | COMPLEX-I | - |
dc.subject.keywordPlus | GLUTAMATE NEUROTOXICITY | - |
dc.subject.keywordPlus | MONOAMINE-OXIDASE | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | INDUCED DAMAGE | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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