Myosin light chain kinase- and PKC-dependent contraction of LES and esophageal smooth muscle
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sohn, UD | - |
dc.contributor.author | Cao, WB | - |
dc.contributor.author | Tang, DC | - |
dc.contributor.author | Stull, JT | - |
dc.contributor.author | Haeberle, JR | - |
dc.contributor.author | Wang, CLA | - |
dc.contributor.author | Harnett, KM | - |
dc.contributor.author | Behar, J | - |
dc.contributor.author | Biancani, P | - |
dc.date.available | 2019-05-30T09:39:10Z | - |
dc.date.issued | 2001-08 | - |
dc.identifier.issn | 0193-1857 | - |
dc.identifier.issn | 1522-1547 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25200 | - |
dc.description.abstract | In smooth muscle cells enzymatically isolated from circular muscle of the esophagus (ESO) and lower esophageal sphincter (LES), ACh-induced contraction and myosin light chain (MLC) phosphorylation were similar. Contraction and phosphorylation induced by purified MLC kinase (MLCK) were significantly greater in LES than ESO. ACh-induced contraction and MLC phosphorylation were inhibited by calmodulin and MLCK inhibitors in LES and by protein kinase C (PKC) inhibitors in ESO. Contraction of LES and ESO induced by the PKC agonist 1,2-dioctanoylglycerol (DG) was unaffected by MLCK inhibitors. Caldesmon and calponin concentration-dependently inhibited ACh-induced contraction of ESO and not LES. In ESO, caldesmon antagonist GS17C reversed caldesmon- but not calponin-induced ACh inhibition. GS17C caused contraction of permeabilized ESO but had much less effect on LES. GS17C-induced contraction was not affected by MLCK inhibitors, suggesting that MLCK may not regulate caldesmon- mediated contraction. DG-induced contraction of ESO and LES was inhibited by caldesmon and calponinin, suggesting that these proteins may regulate PKC-dependent contraction. We conclude that calmodulin and MLCK play a role in ACh-induced LES contraction, whereas the classical MLCK may not be the major kinase responsible for contraction and phosphorylation of MLC in ESO. ESO contraction is PKC dependent. Caldesmon and/or calponin may play a role in PKC-dependent contraction. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER PHYSIOLOGICAL SOC | - |
dc.title | Myosin light chain kinase- and PKC-dependent contraction of LES and esophageal smooth muscle | - |
dc.type | Article | - |
dc.identifier.doi | 10.1152/ajpgi.2001.281.2.G467 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, v.281, no.2, pp G467 - G478 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000169841200020 | - |
dc.identifier.scopusid | 2-s2.0-0034880496 | - |
dc.citation.endPage | G478 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | G467 | - |
dc.citation.title | AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | - |
dc.citation.volume | 281 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | calcium stores | - |
dc.subject.keywordAuthor | caldesmon | - |
dc.subject.keywordAuthor | calmodulin | - |
dc.subject.keywordAuthor | calponin | - |
dc.subject.keywordAuthor | cat | - |
dc.subject.keywordAuthor | second messenger system | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION PATHWAYS | - |
dc.subject.keywordPlus | SPHINCTER CIRCULAR MUSCLE | - |
dc.subject.keywordPlus | CHICKEN GIZZARD | - |
dc.subject.keywordPlus | CALMODULIN-BINDING | - |
dc.subject.keywordPlus | CAT ESOPHAGEAL | - |
dc.subject.keywordPlus | PHOSPHORYLATION SITES | - |
dc.subject.keywordPlus | F-ACTIN | - |
dc.subject.keywordPlus | CALDESMON | - |
dc.subject.keywordPlus | CELLS | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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