TBAK-1 and TASK-1, two-pore K+ channel subunits: kinetic properties and expression in rat heart
DC Field | Value | Language |
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dc.contributor.author | Kim, Yangmi | - |
dc.contributor.author | Bang, Hyoweon | - |
dc.contributor.author | Kim, Donghee | - |
dc.date.available | 2019-05-30T10:35:28Z | - |
dc.date.issued | 1999-11 | - |
dc.identifier.issn | 0363-6135 | - |
dc.identifier.issn | 1522-1539 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25382 | - |
dc.description.abstract | TEAK-I and TASK-1, two-pore K+ channel subunits: kinetic properties and expression in rat heart. Am. J. Physiol. 277 (Heart Circ. Physiol. 46): H1669-H1678, 1999. -A mammalian K+ channel subunit (TBAK-1/TASK-1) containing two pore domains and four transmembrane segments and whose mRNA is highly expressed in the heart has been cloned recently. TEAK-I and TASK-1 are identical except for the additional nine amino acids in the NH2 terminus of TEAK-I. We examined their kinetic properties, pH sensitivity, and regional cardiac mRNA expression and determined whether a native cardiac K+ channel with similar kinetic properties was present. When TBAK-1 or TASK-1 was transiently expressed in COS-7 cells, time- and voltage-independent whole cell currents were observed. Single-channel conductances of TEAK-I and TASK-1 were 14.6 +/- 1.0 and 13.8 +/- 2.8 pS, respectively, at -80 mV in 140 mM extracellular K+, and the mean open times were 0.8 +/- 0.1 and 0.6 +/- 0.1 ms, respectively. Both TBAK-1 and TASK-1 were highly sensitive to extracellular pH such that a decrease from 7.2 to 6.4 reduced their open probability (P-o) by 81 +/- 14% and 80 +/- 16%, whereas a decrease in intracellular pH from 7.2 to 6.4 reduced the P-o by 42 +/- 10% and 47 +/- 12%, respectively. TBAK-1/TASK-1 mRNA was expressed in all regions of the rat heart, with the highest level of expression in the right atrium. A 14-pS K+ channel with kinetic properties similar to those of TBAK-1/TASK-1 was identified in rat atrial and ventricular cells. These results indicate that TBAK-1/TASK-1 represents a functional native K+ channel in the rat heart. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER PHYSIOLOGICAL SOC | - |
dc.title | TBAK-1 and TASK-1, two-pore K+ channel subunits: kinetic properties and expression in rat heart | - |
dc.type | Article | - |
dc.identifier.doi | 10.1152/ajpheart.1999.277.5.h1669 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, v.277, no.5, pp H1669 - H1678 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000083598600004 | - |
dc.identifier.scopusid | 2-s2.0-0032735159 | - |
dc.citation.endPage | H1678 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | H1669 | - |
dc.citation.title | AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | - |
dc.citation.volume | 277 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | two-pore potassium channel | - |
dc.subject.keywordAuthor | atrial cell | - |
dc.subject.keywordAuthor | pH | - |
dc.subject.keywordPlus | POTASSIUM CHANNEL | - |
dc.subject.keywordPlus | FUNCTIONAL EXPRESSION | - |
dc.subject.keywordPlus | ATRIAL CELLS | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | STOICHIOMETRY | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | PH | - |
dc.relation.journalResearchArea | Cardiovascular System & Cardiology | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Cardiac & Cardiovascular Systems | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.relation.journalWebOfScienceCategory | Peripheral Vascular Disease | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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