Proinflammatory effects of High Mobility Group B1 (HMGB1) versus LPS and the mechanism of IL-8 promoter stimulation by HMGB1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeon, Eun Ju | - |
dc.contributor.author | Kwak, Hee Won | - |
dc.contributor.author | Song, Ju Han | - |
dc.contributor.author | Lee, Young Woo | - |
dc.contributor.author | Chung, Jae Woo | - |
dc.contributor.author | Choi, Jae Chul | - |
dc.contributor.author | Shin, Jong Wook | - |
dc.contributor.author | Park, In Won | - |
dc.contributor.author | Choi, Byoung Whui | - |
dc.contributor.author | Kim, Jae Yeol | - |
dc.date.available | 2019-06-10T06:30:46Z | - |
dc.date.issued | 2007-04 | - |
dc.identifier.issn | 1738-3536 | - |
dc.identifier.issn | 2005-6184 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25479 | - |
dc.description.abstract | Background: High mobility group box 1 (HMGB1) is a novel, late mediator of inflammation. This study compared the pro-inflammatory effects of LPS and HMGB1. The transcriptional factors that play an important role in mediating the HMGB1-induced stimulation of IL-8 were also evaluated. Methods: RAW264.7 cells were stimulated with either LPS (100 ng/ml) or HMGB1 (500 ng/ml). The TNF-α, MIP-2 and IL-1β levels in the supernatant were evaluated by ELISA at 0, 2, 4, 8, 12 and 24h after stimulatioa. An acute lung injury was induced by an injection of LPS (5 mg/kg) or HMGB1 (2.5 mg/kg) into the peritoneum of the Balb/c mice. The lung cytokines and MPO activity were measured at 4h (for LPS) or 24h (for HMGB1) after the injection. The transcriptional factor binding sites for NF-IL6, NF-κB and AP-1 in the IL-8 promoter region were artificially mutated. Each mutant was ligated with pIL-6luc and transfected into the RAW264.7 cells. One hour after stimulation with HMGB1 (500 ng/ml), the cell lysate was analyzed for the luciferase activity. Results: The expression of MIP-2, which peaked at 8h with LPS stimulation, increased sequentially until 24h after HMGB1 stimulation. An intraperitoneal injection of HMGB1, which induced a minimal increased in IL-1β expression, provoked the accumulation of neutrophils the lung. A mutation of AP-1 as well as NF-κB in the IL-8 promoter region resulted in a lower ludferase activity after HMGB1 stimulation. Conclusion: The proinflammatory effects of HMGB1, particularly on IL-8, are mediated by both NF-κB and AP-1. | - |
dc.format.extent | 9 | - |
dc.language | 한국어 | - |
dc.language.iso | KOR | - |
dc.publisher | Korean National Tuberculosis Association | - |
dc.title | Proinflammatory effects of High Mobility Group B1 (HMGB1) versus LPS and the mechanism of IL-8 promoter stimulation by HMGB1 | - |
dc.type | Article | - |
dc.identifier.doi | 10.4046/trd.2007.62.4.299 | - |
dc.identifier.bibliographicCitation | Tuberculosis and Respiratory Diseases, v.62, no.4, pp 299 - 307 | - |
dc.identifier.kciid | ART001052845 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-34547406651 | - |
dc.citation.endPage | 307 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 299 | - |
dc.citation.title | Tuberculosis and Respiratory Diseases | - |
dc.citation.volume | 62 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | AP-1 | - |
dc.subject.keywordAuthor | HMGB1 | - |
dc.subject.keywordAuthor | IL-1β | - |
dc.subject.keywordAuthor | LPS | - |
dc.subject.keywordAuthor | MIP-2 | - |
dc.subject.keywordAuthor | MPO | - |
dc.subject.keywordAuthor | NF-κB | - |
dc.subject.keywordAuthor | TNF-α | - |
dc.subject.keywordPlus | CCAAT enhancer binding protein delta | - |
dc.subject.keywordPlus | cytokine | - |
dc.subject.keywordPlus | high mobility group B1 protein | - |
dc.subject.keywordPlus | immunoglobulin enhancer binding protein | - |
dc.subject.keywordPlus | interleukin 1beta | - |
dc.subject.keywordPlus | interleukin 8 | - |
dc.subject.keywordPlus | lipopolysaccharide | - |
dc.subject.keywordPlus | luciferase | - |
dc.subject.keywordPlus | macrophage inflammatory protein 2 | - |
dc.subject.keywordPlus | myeloperoxidase | - |
dc.subject.keywordPlus | transcription factor | - |
dc.subject.keywordPlus | transcription factor AP 1 | - |
dc.subject.keywordPlus | tumor necrosis factor alpha | - |
dc.subject.keywordPlus | acute lung injury | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | animal experiment | - |
dc.subject.keywordPlus | animal model | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | binding site | - |
dc.subject.keywordPlus | cell lysate | - |
dc.subject.keywordPlus | cell stimulation | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | drug effect | - |
dc.subject.keywordPlus | enzyme activity | - |
dc.subject.keywordPlus | enzyme assay | - |
dc.subject.keywordPlus | enzyme linked immunosorbent assay | - |
dc.subject.keywordPlus | inflammation | - |
dc.subject.keywordPlus | leukocyte activation | - |
dc.subject.keywordPlus | mouse | - |
dc.subject.keywordPlus | mutation | - |
dc.subject.keywordPlus | neutrophil | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | peritoneum | - |
dc.subject.keywordPlus | promoter region | - |
dc.subject.keywordPlus | protein expression | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.