RNase E maintenance of proper FtsZ/FtsA ratio required for nonfilamentous growth of Escherichia coli cells but not for colony-forming ability
DC Field | Value | Language |
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dc.contributor.author | Tamura, Masaru | - |
dc.contributor.author | Lee, Kangseok | - |
dc.contributor.author | Miller, Christine A. | - |
dc.contributor.author | Moore, Christopher J. | - |
dc.contributor.author | Shirako, Yukio | - |
dc.contributor.author | Kobayashi, Masahiko | - |
dc.contributor.author | Cohen, Stanley N. | - |
dc.date.available | 2019-06-10T08:30:51Z | - |
dc.date.issued | 2006-07 | - |
dc.identifier.issn | 0021-9193 | - |
dc.identifier.issn | 1098-5530 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25564 | - |
dc.description.abstract | Inactivation or deletion of the RNase E-encoding rne gene of Escherichia coli results in the growth of bacterial cells as filamentous chains in liquid culture (K. Goldblum and D. Apirion, J. Bacteriol. 146:128-132, 1981) and the loss of colony-forming ability (CFA) on solid media. RNase E dysfunction is also associated with abnormal processing of ftsQAZ transcripts (K. Cam, G. Rome, H. M. Krisch, and J.-P. Bouché, Nucleic Acids Res. 24:3065-3070, 1996), which encode proteins having a central role in septum formation during cell division. We show here that RNase E regulates the relative abundances of FtsZ and FtsA proteins and that RNase E depletion results in decreased FtsZ, increased FtsA, and consequently an altered FtsZ/FtsA ratio. However, while restoration of the level of FtsZ to normal in rne null mutant bacteria reverses the filamentation phenotype, it does not restore CFA. Conversely, overexpression of a related RNase, RNase G, in rne-deleted bacteria restores CFA, as previously reported, without affecting FtsZ abundance. Our results demonstrate that RNase E activity is required to maintain a proper cellular ratio of the FtsZ and FtsA proteins in E. coli but that FtsZ deficiency does not account for the nonviability of cells lacking RNase E. Copyright © 2006, American Society for Microbiology. All Rights Reserved. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Society for Microbiology | - |
dc.title | RNase E maintenance of proper FtsZ/FtsA ratio required for nonfilamentous growth of Escherichia coli cells but not for colony-forming ability | - |
dc.type | Article | - |
dc.identifier.doi | 10.1128/JB.00367-06 | - |
dc.identifier.bibliographicCitation | Journal of Bacteriology, v.188, no.14, pp 5145 - 5152 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000239079300014 | - |
dc.identifier.scopusid | 2-s2.0-33745885282 | - |
dc.citation.endPage | 5152 | - |
dc.citation.number | 14 | - |
dc.citation.startPage | 5145 | - |
dc.citation.title | Journal of Bacteriology | - |
dc.citation.volume | 188 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | DIVISION PROTEIN FTSZ | - |
dc.subject.keywordPlus | 16S RIBOSOMAL-RNA | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | RIBONUCLEASE-E | - |
dc.subject.keywordPlus | PROCESSING ENZYME | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | DEGRADOSOME | - |
dc.subject.keywordPlus | MATURATION | - |
dc.subject.keywordPlus | MUTATION | - |
dc.subject.keywordPlus | ENCODES | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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