Myocardial protection by recombinant soluble P-selectin glycoprotein ligand-1: Suppression of neutrophil and platelet interaction following ischemia and reperfusion
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ham, Sang Soo | - |
dc.contributor.author | Jang, Yoon Young | - |
dc.contributor.author | Song, Jin Ho | - |
dc.contributor.author | Lee, Hyang Mi | - |
dc.contributor.author | Kim, Kwang Joon | - |
dc.contributor.author | Hong, Jun Sik | - |
dc.contributor.author | Shin, Yong Kyoo | - |
dc.date.available | 2019-06-11T08:34:27Z | - |
dc.date.issued | 2000-12 | - |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.issn | 2093-3827 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25680 | - |
dc.description.abstract | Polymorphonuclear leukocytes (PMNs) play an important role in myocardial ischemia/reperfusion (MI/R) injury. Moreover, platelets are also important blood cells that can aggravate myocardial ischemic injury. This study was designed to test the effects of PMNs and platelets separately and together in provoking cardiac dysfunction in isolated perfused rat hearts following ischemia and reperfusion. Additional control rat hearts were perfused with 75 × 106 PMNs, with 75 × 106 platelets, or with 75 × 106 PMNs + 75 × 106 platelets over a five minute perfusion followed by a 75 min observation period. No significant reduction in coronary flow (CF), left ventricular developed pressure (LVDP), or the first derivative of LVDP (dP/dt max) was observed at the end of the observation period in any non-ischemic group. Similarly, global ischemia (I) for 20 min followed by 45 minutes of reperfusion (R) produced no sustained effects on the final recovery of any of these parameters in any group of hearts perfused in the absence of blood cells. However, I/R hearts perfused with either PMNs or platelets alone exhibited decreases in these variables of 5 ∼ 10% (p < 0.05 from control). Furthermore, I/R hearts perfused with both PMNs and platelets exhibited decreases of 50 to 60% in all measurements of cardiac function (p < 0.01). These dual cell perfused I/R hearts also exhibited marked increases in cardiac myeloperoxidase (MPO) activity indicating a significant PMN infiltration, and enhanced P-selectin expression on the coronary microvascular endothelium. All cardiaodynamic effects as well as PMN accumulation and P-selectin expression were markedly attenuated by a recombinant soluble PSGL-1 which inhibits selectin mediated cell adhesion. These results provide evidence that platelets and PMNs act synergistically in provoking post-reperfusion cardiac dysfunction, and that this may be largely due to cell to cell interactions mediated by P-selectin. These results also demonstrate that a recombinant soluble PSGL-1 reduces myocardial reperfusion injury by platelet and PMNs interaction. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 대한약리학회 | - |
dc.title | Myocardial protection by recombinant soluble P-selectin glycoprotein ligand-1: Suppression of neutrophil and platelet interaction following ischemia and reperfusion | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | The Korean Journal of Physiology & Pharmacology, v.4, no.6, pp 515 - 523 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-0034519150 | - |
dc.citation.endPage | 523 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 515 | - |
dc.citation.title | The Korean Journal of Physiology & Pharmacology | - |
dc.citation.volume | 4 | - |
dc.identifier.url | https://www.kjpp.net/journal/view.html?uid=304&vmd=Full | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Cardiac dysfunction | - |
dc.subject.keywordAuthor | Ischemia and reperfusion | - |
dc.subject.keywordAuthor | Neutrophils | - |
dc.subject.keywordAuthor | P-selectin | - |
dc.subject.keywordAuthor | Platelets | - |
dc.subject.keywordPlus | myeloperoxidase | - |
dc.subject.keywordPlus | P selectin glycoprotein ligand 1 | - |
dc.subject.keywordPlus | PADGEM protein | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | animal tissue | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | cell infiltration | - |
dc.subject.keywordPlus | cell interaction | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | coronary artery blood flow | - |
dc.subject.keywordPlus | coronary blood vessel | - |
dc.subject.keywordPlus | enzyme activity | - |
dc.subject.keywordPlus | heart disease | - |
dc.subject.keywordPlus | heart function | - |
dc.subject.keywordPlus | heart muscle ischemia | - |
dc.subject.keywordPlus | heart muscle reperfusion | - |
dc.subject.keywordPlus | heart perfusion | - |
dc.subject.keywordPlus | heart protection | - |
dc.subject.keywordPlus | isolated heart | - |
dc.subject.keywordPlus | leukocyte | - |
dc.subject.keywordPlus | male | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | rat | - |
dc.subject.keywordPlus | reperfusion injury | - |
dc.subject.keywordPlus | thrombocyte activation | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.