MAPK activation and cell viability after H2O2 stimulation in cultured feline ileal smooth muscle cells
DC Field | Value | Language |
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dc.contributor.author | Song, H.J. | - |
dc.contributor.author | Jeong, J.H. | - |
dc.contributor.author | Lee, D.K. | - |
dc.contributor.author | Lee, T.S. | - |
dc.contributor.author | Min, Y.S. | - |
dc.contributor.author | Sohn, U.D. | - |
dc.date.available | 2019-06-26T01:24:08Z | - |
dc.date.issued | 2004-12 | - |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.issn | 2093-3827 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/26221 | - |
dc.description.abstract | Recent data have shown the importance of oxidative stresses in the pathogenesis of inflammatory bowel disease, crohn's disease and ulcerative colitis. H2O2, reactive oxygen species (ROS) donor, has been reported to act as a signaling molecule involved in a variety of cellular functions such as apoptosis and proliferation. In the present study, we investigated viability of cultured ileal smooth muscle cells (ISMC) after stimulation with H2O2. Trypan blue method revealed that the cell viability of ISMC treated with 1 mM H2O2 was not different from that of controls at up to 2 h time point, while treatment of ISMC with 1 mM H2O2 for 48 h finally induced significant decrease in the cell viability. Therefore, we evaluated whether H2O2 was capable of ERKs activation in ISMC for the short-term exposure and examined whether tyrosine kinase was involved in the process of ERK activation by H2O2 in ISMC. We also investigated the effects of H2O2 on activation of SAPK/JNK and p38 MAP kinase in ISMC. Thus, ISMC were cultured and exposed to H2O2 and western blot analysis was performed with phosphospecific MAP kinase antibodies. Robust activation of ERK occurred within 30 min of 1 mM H2O2 treatment. H2O2-induced ERK activation was attenuate y a tyrosine kinase inhibitor, genistein, indicating that tyrosine kinase was probably involved in the ERK activation by H2O2. H2O2 was a moderate activator of SAPK/JNK, while p38 MAP kinase was not activated by H2O2. We suggest that ERK activation induced by short-term H2O2 treatment plays a critical role in cellular protection in the early stage of response to oxidative stress. The present study suggests the necessity of identification of MAPK signaling pathways affected by ROS, since it could ultimately elucidate cellular consequences involved in initiation and perpetuation of intestinal tissue damage in the diseases such as crohn's disease and ulcerative colitis, resulted from excessive ROS. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 대한약리학회 | - |
dc.title | MAPK activation and cell viability after H2O2 stimulation in cultured feline ileal smooth muscle cells | - |
dc.title.alternative | MAPK Activation and Cell Viability after H2O2 Stimulation in Cultured Feline lleal Smooth Muscle Cells | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | Korean Journal of Physiology and Pharmacology, v.8, no.6, pp 339 - 344 | - |
dc.identifier.kciid | ART001142470 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-11344266547 | - |
dc.citation.endPage | 344 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 339 | - |
dc.citation.title | Korean Journal of Physiology and Pharmacology | - |
dc.citation.volume | 8 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Cell viability | - |
dc.subject.keywordAuthor | ERKs | - |
dc.subject.keywordAuthor | H2O2 | - |
dc.subject.keywordAuthor | Ileal smooth muscle cells | - |
dc.subject.keywordAuthor | SAPK/JNK | - |
dc.subject.keywordPlus | enzyme antibody | - |
dc.subject.keywordPlus | genistein | - |
dc.subject.keywordPlus | hydrogen peroxide | - |
dc.subject.keywordPlus | mitogen activated protein kinase | - |
dc.subject.keywordPlus | mitogen activated protein kinase p38 | - |
dc.subject.keywordPlus | protein tyrosine kinase | - |
dc.subject.keywordPlus | protein tyrosine kinase inhibitor | - |
dc.subject.keywordPlus | reactive oxygen metabolite | - |
dc.subject.keywordPlus | stress activated protein kinase | - |
dc.subject.keywordPlus | trypan blue | - |
dc.subject.keywordPlus | adolescent | - |
dc.subject.keywordPlus | animal tissue | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | cat | - |
dc.subject.keywordPlus | cell protection | - |
dc.subject.keywordPlus | cell stimulation | - |
dc.subject.keywordPlus | cell viability | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | Crohn disease | - |
dc.subject.keywordPlus | enzyme activation | - |
dc.subject.keywordPlus | female | - |
dc.subject.keywordPlus | ileum | - |
dc.subject.keywordPlus | intestine muscle | - |
dc.subject.keywordPlus | male | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | oxidative stress | - |
dc.subject.keywordPlus | signal transduction | - |
dc.subject.keywordPlus | smooth muscle fiber | - |
dc.subject.keywordPlus | time | - |
dc.subject.keywordPlus | tissue injury | - |
dc.subject.keywordPlus | ulcerative colitis | - |
dc.subject.keywordPlus | Western blotting | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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