요로감염 대장균의 병원성 유전자 분석 및 Rep-PCR Genomic Fingerprinting
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 허준 | - |
dc.contributor.author | 김기정 | - |
dc.contributor.author | 송미옥 | - |
dc.contributor.author | 박철민 | - |
dc.contributor.author | 김원용 | - |
dc.contributor.author | 정상인 | - |
dc.date.available | 2019-06-26T01:24:35Z | - |
dc.date.issued | 2003-03 | - |
dc.identifier.issn | 1598-2467 | - |
dc.identifier.issn | 2093-0429 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/26234 | - |
dc.description.abstract | The profile of virulence genes and repetitive element sequence-based PCR (rep-PCR) genomic fingerprinting were determined on Escherichia coli strains isolated from patients with urinary tract infection to investigate genetic relatedness and its identification. Thirty nine strains of E. coli were examined genotypically by using the multiplex polymerase chain reaction for the presence of five urovirulence genes; pyelonephritis-associated pili (pap), S. fimbriae (sfa), afimbrial adhesin (afa), cytotoxic necrotizing factor (cnf), and α-hemolysin (hly). As a result, genotype pap+sfa -afa-cnf-hly- was the most dominant (14 strains: 36%). But no urovirulence-genes were detected in 12 strains (31%). On the basis of rep-PCR, the dendrograms generated from REP-PCR and ERIC-PCR revealed that uropathogenic E. coli strains were clustered into non-uropathogenic E. coli ATCC 43894 O157:H7 with the degree of similarity 37% and 44%, respectively. On the contrary, BOX-PCR results showed that uropathogenic E. coli strains differed from non-uropathogenic E. coli ATCC 43894 O157:H7 with the degree of similarity 37%. According to these findings, REP-PCR and ERIC-PCR were unable to discriminate reliably uropathogenic E. coli from non-uropathogenic E. coli. However, BOX-PCR provided an effective mean of differentiating E. coli strains between uropathogenic and non-uropathogenic. | - |
dc.format.extent | 10 | - |
dc.language | 한국어 | - |
dc.language.iso | KOR | - |
dc.publisher | The Korean Society for Mocrobiology / The Korean Society of Virology | - |
dc.title | 요로감염 대장균의 병원성 유전자 분석 및 Rep-PCR Genomic Fingerprinting | - |
dc.title.alternative | Profile of Virulence Genes and Rep-PCR Genomic Fingerprinting on Escherichia coli Strains Isolated from Patients with Urinary Tract Infection | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | Journal of Bacteriology and Virology, v.33, no.1, pp 1 - 10 | - |
dc.identifier.kciid | ART001188375 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-24044534758 | - |
dc.citation.endPage | 10 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | Journal of Bacteriology and Virology | - |
dc.citation.volume | 33 | - |
dc.identifier.url | https://kiss.kstudy.com/Detail/Ar?key=2392372 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Genomic fingerprinting | - |
dc.subject.keywordAuthor | Rep-PCR | - |
dc.subject.keywordAuthor | Uropathogenic E. coli | - |
dc.subject.keywordPlus | adhesin | - |
dc.subject.keywordPlus | hemolysin | - |
dc.subject.keywordPlus | amino acid sequence | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | bacterial gene | - |
dc.subject.keywordPlus | bacterial virulence | - |
dc.subject.keywordPlus | bacterium isolation | - |
dc.subject.keywordPlus | DNA fingerprinting | - |
dc.subject.keywordPlus | Escherichia coli | - |
dc.subject.keywordPlus | genetic identification | - |
dc.subject.keywordPlus | genome analysis | - |
dc.subject.keywordPlus | genotype | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | polymerase chain reaction | - |
dc.subject.keywordPlus | strain difference | - |
dc.subject.keywordPlus | urinary tract infection | - |
dc.subject.keywordPlus | Escherichia coli | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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