Standardization and seasonal variation of quercetin glycoside in Eucommiae folium

Abstract

The radix and folium of Eucommia ulmoides(Eucommiaceae) has been used for backache, atrophy of the leg and knee, enuresis, hypertension. In order to evaluate to quality of folium, we have been isolated a main compound as a standard compound, which was elucidated quercetin-3-O-β-D-xylopyranosyl(1→2)-β-D-glucopyranoside through it's physicochemical data and IR, FAB-Mass, 13C-NMR and 1H-NMR analysis. It was analyzed by HPLC system using 17% CH3CN as a solvent system. The amount of quercetin-3-O-β-D-xylopyranosyl(1→2)-β-D-glucopyranoside from Eucommiae folium was in the range of 0.056±0.022 mg(n=7) and also from the results of analysis through seasonal variation(June-October) September and October have been evaluated to be very high in it's content.