Molecular cloning and characterization of an antigenic protein with a repeating region from Clonorchis sinensis.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, T.Y. | - |
dc.contributor.author | Kang, S.Y. | - |
dc.contributor.author | Ahn, I.Y. | - |
dc.contributor.author | Cho, S.Y. | - |
dc.contributor.author | Hong, S.J. | - |
dc.date.available | 2019-06-26T01:27:54Z | - |
dc.date.issued | 2001-03 | - |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.issn | 1738-0006 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/26303 | - |
dc.description.abstract | In the course of immunoscreening of Clonorchis sinensis cDNA library, a cDNA CsRP12 containing a tandem repeat was isolated. The cDNA CsRP12 encodes two putative peptides of open reading frames (ORFs) 1 and 2 (CsRP12-1 and -2). The repetitive region is composed of 15 repeats of 10 amino acids. Of the two putative peptides, CsRP12-1 was proline-rich and found to have homologues in several organisms. Recombinant proteins of the putative peptides were bacterially produced and purified by an affinity chromatography. Recombinant CsRP12-1 protein was recognized by sera of clonorchiasis patients and experimental rabbits, but recombinant CsRP12-2 was not. One of the putative peptide, CsRP12-1, is designated CsPRA, proline-rich antigen of C. sinensis. Both the C-termini of CsRP12-1 and -2 were bacterially produced and analysed to show no antigenicity. Recombinant CsPRA protein showed high sensitivity and specificity. In experimental rabbits, IgG antibodies to CsPRA was produced between 4 and 8 weeks after the infection and decreased thereafter over one year. These results indicate that CsPRA is equivalent to a natural protein and a useful antigenic protein for serodiagnosis of human clonorchiasis. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.title | Molecular cloning and characterization of an antigenic protein with a repeating region from Clonorchis sinensis. | - |
dc.type | Article | - |
dc.identifier.doi | 10.3347/kjp.2001.39.1.57 | - |
dc.identifier.bibliographicCitation | The Korean journal of parasitology, v.39, no.1, pp 57 - 66 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-0035292728 | - |
dc.citation.endPage | 66 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 57 | - |
dc.citation.title | The Korean journal of parasitology | - |
dc.citation.volume | 39 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordPlus | helminth DNA | - |
dc.subject.keywordPlus | parasite antigen | - |
dc.subject.keywordPlus | recombinant protein | - |
dc.subject.keywordPlus | amino acid sequence | - |
dc.subject.keywordPlus | animal | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | Clonorchis sinensis | - |
dc.subject.keywordPlus | gene library | - |
dc.subject.keywordPlus | genetics | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | immunology | - |
dc.subject.keywordPlus | isolation and purification | - |
dc.subject.keywordPlus | molecular cloning | - |
dc.subject.keywordPlus | molecular genetics | - |
dc.subject.keywordPlus | nucleotide repeat | - |
dc.subject.keywordPlus | nucleotide sequence | - |
dc.subject.keywordPlus | rabbit | - |
dc.subject.keywordPlus | Amino Acid Sequence | - |
dc.subject.keywordPlus | Animals | - |
dc.subject.keywordPlus | Antigens, Helminth | - |
dc.subject.keywordPlus | Base Sequence | - |
dc.subject.keywordPlus | Cloning, Molecular | - |
dc.subject.keywordPlus | Clonorchis sinensis | - |
dc.subject.keywordPlus | DNA, Helminth | - |
dc.subject.keywordPlus | Gene Library | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Molecular Sequence Data | - |
dc.subject.keywordPlus | Rabbits | - |
dc.subject.keywordPlus | Recombinant Proteins | - |
dc.subject.keywordPlus | Repetitive Sequences, Nucleic Acid | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.